Functional Analysis of Secreted Protein MoCDIE2 Inducing Plant Cell-Death in Magnaporthe oryzae

doi:10.13560/j.cnki.biotech.bull.1985.2019-0606

Abstract

Abstract: Rice blast,caused by the fungus Magnaporthe oryzae,is one of the most destructive plant diseases around the world. M. oryzae secretes many effector proteins into the interface or plant cells to suppress host immune defense response,thus helping the fungus to infect plant successfully. Using agrobacterium-mediated transient expression strategy,we obtained one putative effector protein MoCDIE2（Cell Death-Inducing Effector）that induces cell death in non-host plant Nicotiana benthamiana. Sequence alignment result indicated that MoCDIE2 gene encoded a ricin B lectin protein. Phylogenetic tree demonstrated that MoCDIE2 protein was conserved in many filamentous fungi. We used gene knock out method to generate MoCDIE2 knock out mutant,and the result showed that there were no significant differences between wild type strain Guy11 and mutants of MoCDIE2 knockout in mycelia growth and pathogenicity.

Key words: Magnaporthe oryzae, MoCDIE2, gene knockout, ricin B lectin protein

WU Hang, LI Zhi-qiang, LIU Wen-de. Functional Analysis of Secreted Protein MoCDIE2 Inducing Plant Cell-Death in Magnaporthe oryzae[J]. Biotechnology Bulletin, 2020, 36(1): 15-22.

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