Abstract: Multiple traits of crops such as rice and corn had been improved in single time of transformation by the strategy of multiple gene transformation. For exploring the feasibility of multiple gene transformation of sugarcane in this research, a multiple gene expression vector was used and transformed to sugarcane and many transformed plants were produced. Multiple PCR method was used to analyze the integration and deficiency of multiple genes in the transformed plants. Results showed that multiple gene transformation of sugarcane was feasible. But due to the insertion of large DNA fragments some genes were lost during the integration. Thus it is a need to have sufficient transformed plants then valuable transformed plants can be selected for further research.

Key words: sugarcane, Agrobacterium-mediated, multiple gene transformation, multiple PCR detection