Biotechnology Bulletin ›› 2016, Vol. 32 ›› Issue (7): 48-53.doi: 10.13560/j.cnki.biotech.bull.1985.2016.07.007

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The Application of 454 High-throughput Sequencing Technology into Anlaysing the Diversity of Soil Fungi in the Field Planting Tamarix chinensis and Angiospermae

WANG Yan-yun GUO Du-fa   

  1. College of Geography and Environment,Shandong Normal University,Ji’nan 250000
  • Received:2015-10-30 Online:2016-07-25 Published:2016-07-25

Abstract: The study,which was related to the variety and diversity of soil fungi in the field planting Tamarix chinensis and Angiospermae,and the corresponding analysis of fungal diversity index and similarity were conducted by applying 454 high-throughput sequencing technology. It is aimed at providing the basic datas and theoretical gist for the futher study into the diversity of fungi in Yellow River Delta and the improvement of saline-alkali soil. And the result indicated four soil samples were divided into 5 phyla,17 classes,37 orders,48 families and 51 genus. The OTU number and diversity index showed that the diversity of soil fungal diversity was the richest in Tamarix chinensis surface(C1:0-20 cm),which was accordingly 1.7 times,1.2 times and 2.4 times of OTU number,Shannon index and Chao1 index in Angiospermae substratum(Z2:20-40 cm). In addition,the analysis of the distance heat map indicated the fungal communities of Z1 and Z2 had the most similarity,whose Unifrac metric value was 0.269 9 and the fungal communities of C1 and C2 have the least similarity,whose value was 0.690 2. In a word,the soil fungi in the field planting Tamarix chinensis was richer than one in the field planting Angiospermae.

Key words: The Yellow River Delta, Tamarix chinensis, Angiospermae, fungi, fungal diversity