Effects of Ralstonia solanacearum Infection on Soil Fungal Community Diversity

doi:10.13560/j.cnki.biotech.bull.1985.2021-1595

Abstract

Abstract:

The soil fungal community plays an important role in maintaining soil fertility and plant health，however，the effect of bacterial wilt from Ralstonia solanacearum infection on the soil fungal community still is little. Real-time quantitative PCR and high-throughput sequencing technology of MiSeq were used to study the fungal community composition in the bulk and rhizosphere soils cropped with diseased and non-diseased tomato. The results showed that the occurrence of R. solanacearum infection significantly affected the fungal community composition of bulk and rhizosphere soil. Compared to the non-diseased tomato rhizosphere soil，the fungal abundance，alpha diversity of Chao1 and Shannon in the diseased tomato rhizosphere soil significantly reduced. And the relative abundances of indigenous beneficial fungi such as Gliocladium，Mortierella，Acremonium and Trichoderma significantly reduced whereas the relative abundance of the harmful fungus of Fusarium significantly increased. In summary，the occurrence of R. solanacearum infection altered the composition of the soil fungal community. The fungal abundance，alpha diversity，and the relative abundance of indigenous beneficial fungi depleted while the relative abundance of harmful fungi enriched. The results may provide the elucidation of the microecological mechanism of outbreak of soil-borne bacterial wilt.

Key words: Ralstonia solanacearum, fungal abundance, fungal diversity, community composition

LI Ting-ting, DENG Xu-hui, LI Ruo-chen, LIU Hong-jun, SHEN Zong-zhuan, LI Rong, SHEN Qi-rong. Effects of Ralstonia solanacearum Infection on Soil Fungal Community Diversity[J]. Biotechnology Bulletin, 2022, 38(10): 195-203.

Figures/Tables 6

Fig.1 Fungal abundance in bulk and rhizosphere soils cropped with diseased and non-diseased tomato BD：Diseased tomato bulk soil；BH：non-diseased tomato bulk soil；RD：diseased tomato rhizosphere soil；RH：non-diseased tomato rhizosphere soil. ***，**，and * indicate that there are significant differences between the treatments at the levels of P <0.001，P <0.01，and P <0.05. The same below

Fig.2 Diversity index for fungal communities in the bulk and rhizosphere soils cropped with diseased and non-diseased tomato

Fig.3 Principal coordinate analysis（PCoA）for fungal communities in the bulk and rhizosphere soils cropped with diseased and non-diseased tomato

Fig.4 Relative abundances of dominated orders for fungal communities in the bulk（a）and rhizosphere soils crop-ped with diseased and non-diseased tomato（b）

Fig.5 Venn plot of fungal composition (a) and relative abu-ndances heatmap (b) of partial dominated genera for fungal communities in the bulk and rhizosphere soils cropped with diseased and non-diseased tomato The colors in the heat map cell indicate the relative abundances of the genera in the soil. The clustering of the heat map is based on the relative abundances of the genera in each sample

Fig. 6 Volcano plot of fungal OTUs in the bulk（a）and rhizosphere soils（b）cropped with diseased and non-diseased soils tomato The gray dot in the figure indicates that the OTU has no significant difference between the diseased and non-diseased treatments. The red dot indicates that the OTU significantly increased in the diseased treatment，and the blue dot indicates that the OTU significantly reduced in the diseased treatment

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