Expression of CydDC in Escherichia coli and the Effect of It on Glutathione Transportation

doi:10.13560/j.cnki.biotech.bull.1985.2016.11.033

Abstract

Abstract: This study is to co-express the CydDC and GshF in the recombinant Escherichia coli,and to increases the glutathione production by enhancing glutathione transportation. As the results from the experiments,the target protein CydDC and GshF were successfully expressed,the glutathione production of MG1655（pTrc99a-as/pBAD33-cydDC）reached 0.22 mmol/L,and the content of glutathione in supernatant significantly increased and reached 81.9%,which was 1.11-fold and 1.29-fold of control strain MG1655（pTrc99a-as/pBAD33）,respectively.

Key words: glutathione, transporter, co-expression, recombinant Escherichia coli

QUAN Cong, ZHANG Jing, WU Hui, LI Zhi-min, YE Qin. Expression of CydDC in Escherichia coli and the Effect of It on Glutathione Transportation[J]. Biotechnology Bulletin, 2016, 32(11): 255-260.

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