Analysis of Expression Characteristics of CmABCG8 Gene in Cucumis melo L.

doi:10.13560/j.cnki.biotech.bull.1985.2021-1117

Abstract

Abstract:

ABC transporter is a kind of proteins with extensive functions,which can actively mediate the transport of various molecules,but its study in melon（Cucumis melo L.）is still unclear. In this paper,the characteristics of ABC transporter subfamily G member CmABCG8 were analyzed by bioinformatics method,and the expression characteristics of CmABCG8 in different fruits and tissues at different periods and their responses to stress were detected by fluorescence quantitative PCR. The phylogenetic tree results showed that CmABCG8 had the closest relationship with cucumber（Cucumis sativus L.）CsABCG23. CmABCG8 was expressed in all tissues of melon,and was highly expressed in the gynoecium and leaves. CmABCG8 had 10 interacting proteins. The results of RT-qPCR showed that the expression of CmABCG8 was the highest at 10 d. CmABCG8 expression was the highest in the leaves among different tissues of melon. After treatment with cytokinin,brassinosteroid and hydrogen peroxide,the expression of CmABCG8 presented an upward trend with the increase of the treatment concentration. After treatment with Fe²⁺,Cu²⁺ and Mn²⁺,the expression of CmABCG8 showed a downward trend. Therefore,it was analyzed that CmABCG8 had a certain influence on the early development of melon fruit and had different degrees of response to plant hormones and metal ions. Plant hormones could effectively induce the expression of CmABCG8.

Key words: Cucumis melo L., ABC transporter, plant hormone, metal ion

HONG Tian-shu, HAI Ying, ENHE Ba-ya-er, GAO Feng. Analysis of Expression Characteristics of CmABCG8 Gene in Cucumis melo L.[J]. Biotechnology Bulletin, 2022, 38(7): 178-185.

Figures/Tables 7

Fig.1 Phylogenetic trees of ABCG genes in CmABCG8 and Arabidopsis thaliana,tomato（Solanum lycopersic-um）,cucumber（Cucumis sativus L.）and melon（Cucumis melo L.）

Fig. 2 Expression of ABCG gene family in the different tissues of melon

Fig.3 Protein interaction network of CmABCG8 in melon

Fig.4 Relative expressions of CmABCG8 gene in melon fruit at different development stages

Fig.5 Relative expressions of CmABCG8 gene in the different tissues of melon The error line in the figure refers to the standard deviation;and * indicates significant difference（*P<0.05 and ** P <0.01）. The same below

Fig.6 Effects of plant hormones and H2O2 treatment on the expression characteristics of CmABCG8 gene A：Cytokinin treatment. B：Brassinosteroid treatment. C：Hydrogen peroxide treatment

Fig.7 Effects of different metal ion treatment on the expression characteristics of CmABCG8 gene A：Fe2+ treatment. B：Cu2+ treatment. C：Mn2+ treatment

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