Microbial Remediation of Soil Heavy Metal and Organic Pollutants：Bioaugmentation and Biostimulation

doi:10.13560/j.cnki.biotech.bull.1985.2017-0294

Abstract

Abstract: Green technologies，e.g.，bioaugmentation and biostimulation，are cost effective and eco-friendly，and are becoming Rosetta Stone for the remediation of soil heavy metal and organic pollutants. Various bacteria，fungi and their consortia can be combined with physical and chemical techniques for the targeted remediation of soil organic contamination，heavy metal contamination and combined pollutions. This review exemplifies the representative studies within the recent five years，summarizes the screening of microbial strains from multiple sources and their potentials in transforming/degrading various types of pollutants，and highlights their applications in the case studies of laboratory，greenhouse and field conditions. The complexity of microbial remediation is ascribed to not only the variations of physiological and metabolic traits，but also numerous environmental factors，including abiotic factors，e.g.，pH，temperature，type of soil，pollutant concentration，content of water and organic matter，additional carbon and nitrogen sources，and biotic factors，e.g.，inoculum size，interactions between the introduced strains and autochthonous microbes，and the survival of inoculants. Prospects of combined remediation and the applications of molecular methods are also presented.

Key words: soil, microbial remediation, organic pollutant, heavy metal, combined remediation

HAO Da-cheng, ZHOU Jian-qiang, HAN Jun. Microbial Remediation of Soil Heavy Metal and Organic Pollutants：Bioaugmentation and Biostimulation[J]. Biotechnology Bulletin, 2017, 33(10): 9-17.

References

[1]Sarwar N, Imran M, Shaheen MR, et al. Phytoremediation strategies for soils contaminated with heavy metals：Modifications and future perspectives[J]. Chemosphere, 2017, 171：710-721.
[2]钱春香, 王明明, 许燕波. 土壤重金属污染现状及微生物修复技术研究进展[J]. 东南大学学报：自然科学版, 2013, 43：669-674.
[3]Mishra GK. Microbes in heavy metal remediation：A review on current trends and patents[J]. Recent Pat Biotechnol, 2017, DOI：10. 2174/1872208311666170120121025.
[4]侯梅芳, 潘栋宇, 黄赛花, 等. 微生物修复土壤多环芳烃污染的研究进展[J]. 生态环境学报, 2014, 23：1233-1238.
[5]Hao DC, Song SM, Mu J, et al. Unearthing microbial diversity of Taxus rhizosphere via MiSeq high-throughput amplicon sequencing and isolate characterization[J]. Sci Rep, 2016, 6：22006.
[6]Cycoń M, Mrozik A, Piotrowska-Seget Z. Bioaugmentation as a strategy for the remediation of pesticide-polluted soil：A review[J]. Chemosphere, 2017, 172：52-71.
[7]Chowdhury A, Pradhan S, Saha M, et al. Impact of pesticides on soil microbiological parameters and possible bioremediation strategies[J]. Ind J Microbiol, 2008, 48：114-127.
[8]Karpouzas DG, Walker A. Factors influencing the ability of Pseudomonas putida epI to degrade ethoprophos in soil[J]. Soil Biol Biochem, 2000, 32：1753-1762.
[9]Singh BK, Walker A, Wright DJ. Bioremedial potential of fenamiphos and chlorpyrifos degrading isolates：influence of different environmental conditions[J]. Soil Biol Biochem, 2006, 38：2682-2693.
[10]Silva VP, Moreira-Santos M, Mateus C, et al. Evaluation of Arthrobacter aurescens strain TC1 as bioaugmentation bacterium in soils contaminated with the herbicidal substance terbuthylazine[J]. PLoS One, 2015, 10：e0144978.
[11]Wang Q, Xie S, Hu R. Bioaugmentation with Arthrobacter sp. strain DAT1 for remediation of heavily atrazine-contaminated soil[J]. Int Biodeterior Biodegr, 2013, 77：63-67.
[12]Das R, Das SJ, Das AC. Effect of synthetic pyrethroid insecticides on N2-fixation and its mineralization in tea soil[J]. Eur J Soil Biol, 2016, 74：9-15.
[13]Cornu JY, Huguenot D, Jézéquel K, et al. Bioremediation of copper-contaminated soils by bacteria[J]. World J Microbiol Biotechnol, 2017, 33(2)：26.
[14]Kuppusamy S, Thavamani P, Venkateswarlu K, et al. Remediation approaches for polycyclic aromatic hydrocarbons(PAHs)contaminated soils：Technological constraints, emerging trends and future directions[J]. Chemosphere, 2017, 168：944-968.
[15]Alvarez A, Saez JM, Davila Costa JS, et al. Actinobacteria：Current research and perspectives for bioremediation of pesticides and heavy metals[J]. Chemosphere, 2017, 166：41-62.
[16]Gao C, Jin X, Ren J, et al. Bioaugmentation of DDT-contaminatedsoil by dissemination of the catabolic plasmid pDOD[J]. J Environ Sci, 2015, 27：42-50.
[17]Zhang Q, Wang B, Cao Z, et al. Plasmid-mediated bioaugmentation for the degradation of chlorpyrifos in soil[J]. J. Hazard Mater, 2012, 221-222：178-184.
[18]Nguyen VK, Tran HT, Park Y, et al. Microbial arsenite oxidation with oxygen, nitrate, or an electrode as the sole electron acceptor[J]. J Ind Microbiol Biotechnol, 2017, 44(6)：857-868.
[19] Pan X, Chen Z, Li L, et al. Microbial strategy for potential lead remediation：a review study[J]. World J Microbiol Biotechnol, 2017, 33(2)：35.
[20] Sun L, Cao X, Li M, et al. Enhanced bioremediation of lead-contaminated soil by Solanum nigrum L. with Mucor circinelloides[J]. Environ Sci Pollut Res Int, 2017, 24(10)：9681-9689.
[21]Abad-Valle P, Iglesias-Jiménez E, álvarez-Ayuso E. A comparative study on the influence of different organic amendments on traceelement mobility and microbial functionality of a polluted mine soil[J]. J Environ Manage, 2017, 188：287-296.
[22]Ali A, Guo D, Mahar A, et al. Phytoextraction of toxic trace elements by Sorghum bicolor inoculated withStreptomycespactum(Act12)in contaminated soils[J]. Ecotoxicol Environ Saf, 2017, 139：202-209.
[23]李韵诗, 冯冲凌, 吴晓芙, 等. 重金属污染土壤植物修复中的微生物功能研究进展[J]. 生态学报, 2015, 35：6881-6890.
[24]Biache C, Ouali S, Cébron A, et al. Bioremediation of PAH-contamined soils：Consequences on formation and degradation of polar-polycyclic aromatic compounds and microbial community abundance[J]. J Hazard Mater, 2017, 329：1-10.
[25]Hao DC, Ge GB, Yang L. Bacterial diversity of Taxus rhizosphere：culture-independent and culture-dependent approaches[J]. FEMS Microbiol Lett, 2008, 284(2)：204-212.
[26]郝大程, 陈士林, 肖培根. 基于分子生物学和基因组学的植物根际微生物研究[J]. 微生物学通报, 2009, 36(6)：892-899.
[27]Ren CG, Kong CC, Bian B, et al. Enhanced phytoremediation of soils contaminated with PAHs by arbuscularmycorrhiza and rhizobium[J]. Int J Phytoremediation, 2017, 19(9)：789-797.
[28]Liang SH, Hsu DW, Lin CY, et al. Enhancement of microbial 2, 4, 6-trinitrotoluene transformation with increased toxicity by exogenous nutrient amendment[J]. Ecotoxicol Environ Saf, 2017, 138：39-46.
[29]Martínez-Pascual E, Grotenhuis T, Solanas AM, et al. Coupling chemical oxidation and biostimulation：Effects on the natural attenuation capacity and resilience of the native microbial community in alkylbenzene-polluted soil[J]. J Hazard Mater, 2015, 300：135-143.
[30]Mandal A, Biswas B, Sarkar B, et al. Surface tailored organobentonite enhances bacterial proliferation and phenanthrene biodegradation under cadmium co-contamination[J]. Sci Total Environ, 2016, 550：611-618.
[31]Biswas B, Sarkar B, Mandal A, et al. Heavy metal-immobilizing organoclay facilitates polycyclic aromatic hydrocarbon biodegradation in mixed-contaminated soil[J]. J Hazard Mater, 2015, 298：129-137.
[32]Chen F, Tan M, Ma J, et al. Efficient remediation of PAH-metal co-contaminated soil using microbial-plant combination：A greenhouse study[J]. J Hazard Mater, 2016, 302：250-261.
[33]Thavamani P, Megharaj M, Naidu R. Metal-tolerant PAH-degrading bacteria：development of suitable test medium and effect of cadmium and its availability on PAH biodegradation[J]. Environ Sci Pollut Res Int, 2015, 22(12)：8957-8968.
[34]Cheng Y, Wang L, Faustorilla V, et al. Integrated electrochemical treatment systems for facilitating the bioremediation of oil spill contaminated soil[J]. Chemosphere, 2017, 175：294-299.
[35]Wu M, Ye X, Chen K, et al. Bacterial community shift and hydrocarbon transformation during bioremediation ofshort-term petroleum-contaminated soil[J]. Environ Pollut, 2017, 223：657-664.
[36]杨茜, 吴蔓莉, 聂麦茜, 等. 石油污染土壤的生物修复技术及微生物生态效应[J]. 环境科学, 2015, 36：1856-1863.
[37]McIntosh P, Schulthess CP, Kuzovkina YA, et al. Bio- and phytoremediation of total petroleum hydrocarbons(TPH)under various conditions[J]. Int J Phytoremediation, 2017, 19(8)：755-764.
[38]Qi Z, Wei Z. Microbial flora analysis for the degradation of beta-cypermethrin[J]. Environ Sci Pollut Res Int, 2017, 24(7)：6554-6562.
[39]Salam JA, Hatha MA, Das N. Microbial-enhanced lindane removal by sugarcane(Saccharum officinarum)in doped soil-applications in phytoremediation and bioaugmentation[J]. J Environ Manage, 2017, 193：394-399.
[40]Li C, Lv T, Liu W, et al. Efficient degradation of chlorimuron-ethyl by a bacterial consortium and shifts in the aboriginal microorganism community during the bioremediation of contaminated-soil[J]. Ecotoxicol Environ Saf, 2017, 139：423-430.
[41]Barba S, Villase?or J, Rodrigo MA, et al. Effect of the polarity reversal frequency in the electrokinetic-biological remediation of oxyfluorfen polluted soil[J]. Chemosphere, 2017, 177：120-127.
[42] Teng Y, Wang X, Zhu Y, et al. Biodegradation of pentachloronitro-benzene by Cupriavidus sp. YNS-85 and its potential for remediation of contaminated soils[J]. Environ Sci Pollut Res Int, 2017, 24(10)：9538-9547.
[43]PerruchonC, Chatzinotas A, Omirou M, et al. Isolation of a bacterial consortium able to degrade the fungicide thiabendazole：the key role of a Sphingomonas phylotype[J]. Appl Microbiol Biotechnol, 2017, 101(9)：3881-3893.
[44]高宪雯. 微生物—植物在石油—重金属复合污染土壤修复中的作用研究[D]. 济南：山东师范大学, 2013.
[45]Chen M, Xu P, Zeng G, et al. Bioremediation of soils contaminated with polycyclic aromatic hydrocarbons, petroleum, pesticides, chlorophenols and heavy metals by composting：Applications, microbes and future research needs[J]. Biotechnol Adv, 2015, 33(6 Pt 1)：745-755.
[46]周建强, 刘晓玲, 韩君, 等. 基于植物仿生的土壤重金属污染原位自持修复技术[J]. 环境化学, 2016, 35(7)：1398-1406.
[47]周建强, 韩君, 徐愿坚, 等. 基于植物仿生的污染土壤原位自持修复中重金属形态变化分析[J]. 环境工程技术学报, 2017, 7：71-77.
[48]周建强. 基于植物仿生的土壤重金属污染原位自持修复技术研究[D]. 重庆：中国科学院重庆绿色智能技术研究院, 2016.
[49]郝大程, 周建强, 王闯, 韩君. 重金属污染土壤的植物仿生和植物修复比较研究[J]. 生物技术通报, 2017, 33(2)：66-71.
[50] Ayaz E, Gothalwal R. Effect of environmental factors on bacterial quorum sensing[J]. Cell Mol Biol(Noisy-le-grand). 2014, 60(5)：46-50.
[51]Wei L, Wang S, Zuo Q, et al. Nano-hydroxyapatite alleviates the detrimental effects of heavy metals on plant growth and soil microbes in e-waste-contaminated soil[J]. Environ Sci Process Impacts. 2016, 18(6)：760-767.
[52]Dueholm MS, Marques IG, Karst SM, et al. Survival and activity of individual bioaugmentation strains[J]. Bioresour Technol, 2015, 186：192-199.
[53]Abbasian F, Palanisami T, Megharaj M, et al. Microbial diversity and hydrocarbon degrading gene capacity of a crude oil field soil as determined by metagenomics analysis[J]. Biotechnol Prog, 2016, 32(3)：638-648.
[54]Garoutte A, Cardenas E, Tiedje J, et al. Methodologies for probing the metatranscriptome of grassland soil[J]. J Microbiol Methods, 2016, 131：122-129.