Abstract: This work is to understand the adsorption characteristics of uranium（U），manganese（Mn），and arsenic（As）to Bacillus licheniformis and the effects of Mn and As on the adsorption of uranium to B. licheniformis，for providing theoretical supports while applying this bacterium in the control of uranium pollution. Enrichment laws of B. licheniformis to uranium，manganese and arsenic，as well as the influences of manganese and arsenic on uranium enrichment were studied by single-factor and multiple-factor experiments. Also，the Fourier Transform Infrared Spectra（FTIR）was used to analyze the influencing mechanism. The research showed that all of them obviously inhibited the growth of B. licheniformis when the concentration of uranium，manganese，and arsenic were over 75 mg/L，25 mg/L，and 25 mg/L，respectively. Meanwhile，the concentration of uranium and manganese was negatively correlated to the adsorption rate respectively when the concentration was in 0-100 mg/L. However，the arsenic adsorption decreased at first and then rose when arsenic concentration was in 0-100 mg/L. The maximum adsorption rate of arsenic reached the highest of 79.73% while its concentration was 100 mg/L. Multiple-factor experiments suggested that arsenic promoted the uranium enrichment of B. licheniformis when its concentration was < 60 mg/L，while inhibition occurred when its concentration was > 60 mg/L. Moreover，manganese at the concentration of < 40 mg/L promoted the enrichment uranium of B. licheniformis，but inhibited when the concentration was > 60 mg/L. Manganese and arsenic affect bacteria on uranium adsorption in form of restraining bacteria growth as well as competing bacteria surface negative groups and converting properties of bacteria.

Key words: Bacillus licheniformis, adsorption, manganese, arsenic, microbial remediation