The Latest Progress on the Methods for in Vitro Screening Aptamers

doi:10.13560/j.cnki.biotech.bull.1985.2017.04.010

Abstract

Abstract: Aptamers are single-stranded oligonucleotides that are screened by systematic evolution of ligands by exponential enrichment（SELEX）in vitro,which can selectively bind to different target with high affinity and high specificity,including protein,small molecules,organic compound,metal ions,drugs,etc. This technology has attracted more attentions for its advantages and thus it has been applied widely in many aspects such as biological sensor,gene chip,new drug development,nano technology,etc. However,the traditional SELEX method is cumbersome,which usually takes several months to screen out the target of nucleic acid in high specificity. With the rapid development of SELEX,many novel screening methods have emerged in recent years. The screening cycle and the screening efficiency are improved with these new methods,and the application of aptamers is expanded. This review introduces several new screening methods in recent three years,including multiple GO-SELEX,SWCNTs-assisted cell-SELEX,on-chip Cell-SELEX,Sequence-constructive SELEX and High-Fidelity（HI FI）SELEX,It is helpful for us to know more about the latest progress on the methods for in vitro screening aptamers and to promote the application of aptamers in various fields..

Key words: aptamer, SELEX, screening method

LI Ya-nan, ZHAO Jie, ZHANG Ao-zhe, TAN Yan, HUA Qian, ZHANG Zi-jian. The Latest Progress on the Methods for in Vitro Screening Aptamers[J]. Biotechnology Bulletin, 2017, 33(4): 78-82.

References

[1] Thiel WH, Thiel KW, Flenker KS, et al. Cell-internalization SELEX：method for identifying cellInternalizing RNA aptamers for delivering siRNAs to target cells[J]. Methods Mol Biol, 2015, 1218：187-199.
[2] 吕彬, 张骏, 杜文琪, 等. Cell-SELEX技术在肿瘤诊治中的应用进展[J]. 复旦学报：医学版, 2013, 40（2）：227-238.
[3] Iliuk AB, Hu LH, Tao WA. Aptamer in bioanalytical applications [J]. Anal Chem, 2011, 83：4440-4452.
[4] 朴喜航, 任姝颖, 夏薇. SELEX技术的研究进展及在医学中的应用[J]. 吉林医药学院学报, 2015, 36：45-49.
[5] 满燕, 吕雪飞, 张玉奎, 等. 核酸适配体及其在生物医学研究中的应用[J]. 航天医学与医学工程, 2013, 26（6）：485-490.
[6] Ozer A, Pagano JM, Lis JT. New technologies provide quantum changes in the scale, speed, and success of SELEX methods and aptamer characterization[J]. Molecular Therapy-Nucleic Acids, 2014, 3：2162-2531.
[7] Kim YS, Gu MB. Advances in aptamer screening and small molecule aptasensors[J]. Adv Biochem Eng Biotechnol, 2014, 140：29-67.
[8] Mayer G. The chemical biology of aptamers[J]. Angew Chem Int Ed, 2009, 48：2672-2689.
[9] Stoltenburg R, Reinemann C, Strehlitz B. FluMag-SELEX as an advantageous method for DNA aptamer selection[J]. Anal Bioanal Chem, 2005, 383：83-91.
[10] Jensen KB, Atkinson BL, Willis MC, et al. Using in vitro selection to direct the covalent attachment of human immunodeficiency virus type 1 Rev protein to high-affinity RNA ligands. Proc[J]. Natl Acad Sci USA, 1995, 92：12220-12224.
[11] Yang Y, Yang DL, Schluesener HJ, et al. Advances in SELEX and application of aptamers in the central nervous system[J]. Biomolecular Engineering, 2007, 24：583-592.
[12] Cox JC, Hayhurst A, Hesselberth J, et al. Automated selection of aptamers against protein targets translated in vitro：from gene to aptamer[J]. Nucleic Acids Res, 2002, 30：e108.
[13] Mendonsa SD, Bowser MT. In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis[J]. Anal Chem, 2004, 76：5387-5392.
[14] Vater A, Jarosch F, Buchner K, et al. Short bioactive Spiegelmers to migraine-associated calcitonin gene-related peptide rapidly identified by a novel approach：Tailored-SELEX[J]. Nucleic Acids Research, 2003, 31：e130.
[15] 沈贺, 张立明, 张智军. 石墨烯在生物医学领域的应用[J]. 东南大学学报：医学版, 2011, 30（1）：219-223.
[16] Wang Y, Li ZH, Wang J, et al. Graphene and grapheneo xide：biofunctionalization and applications in biotechnology[J]. Trends in Biotechnology, 2011, 29：205-212.
[17] Nguyen VT, Kwon YS, Kim JH, et al. Multiple GO-SELEX for efficient screening of flexible aptamers[J]. Chem Commun, 2014, 50：10513-10516.
[18] Gu HJ, Duan N, Wu SJ, et al. Graphene oxide-assisted nonimmob-ilized SELEX of okdaic acid aptamer and the analytical application of aptasensor[J]. Scientific Reports, 2016, 6：21665.
[19] 林彦锋, 刘宇洋, 高顺祥, 等. 氧化石墨烯与核酸适配体在检测方面应用的进展[J]. 中国生化药物杂志, 2015, 35（8）：186-188.
[20] Liang F, Chen B. A review on biomedical applications of single-walled carbon nanotubes[J]. Current Medicinal Chemistry, 2010, 17：10-24.
[21] 李玲. 单壁碳纳米管的改性及其在生物医药领域的初步应用研究[D]. 上海：上海交通大学, 2010.
[22] Tan YY, Guo QP, Xie Q, et al. Single-walled carbon nanotubes（SWCNTs）-assisted cell-systematic evolution of ligands by exponential enrichment（Cell-SELEX）for improving screening efficiency[J]. Anal Chem, 2014, 86（19）：9466-9472.
[23] Guo KT, Paul A, Schichor C, et al. CELL-SELEX：novel perspec-tives of aptamer-based therapeutics[J]. Int J Mol Sci, 2008, 9：668-678.
[24] Hung LY, Wang CH, Hsu KF, et al. An on-chip Cell-SELEX process for automatic selection of high-affinity aptamers specific to different histologically classified ovarian cancer cells[J]. Lab Chip, 2014, 14：4017-4028.
[25] Hung LY, Wang CH, Che YJ, et al. Screening of aptamers specifi to colorectal cancer cells and stem cells by utilizing On-chip Cell-SELEX[J]. Scientific Reports, 2015, 5：10326.
[26] Wang CY, Wu CY, Hung TC, et al. Sequence-constructive SELEX：A new strategy for screening DNA aptamer binding to Globo H, Biochem. Biophys[J]. Res Commun, 2014, 452（3）：484-489.
[27] Ouellet E, Foley JH, Conway EM, et al. Hi-Fi SELEX：a high-fidelity digital-PCR based therapeutic aptamer discovery platform[J]. Biotechnol. Bioeng, 2015, 112：1506-1522.