Synthetic Biology Research of Plant Nitrogen-fixation Organelle

doi:10.13560/j.cnki.biotech.bull.1985.2019-0585

Abstract

Abstract: With the rise of synthetic biology,the long-established field of biological nitrogen fixation has ushered in new development opportunities. After the introduction of the principles and techniques of synthetic biology into nitrogen-fixing biology,a new interdisciplinary subject of nitrogen-fixing synthetic biology was born. Symbiotic nitrogen fixation is the most efficient of the three forms of biological nitrogen fixation. In the symbiotic nitrogen-fixing system,the nitrogen-fixing bacteria stably exist in the cytoplasm of the host plant as the organelles,and the relatively stable nitrogen fixation reaction is carried out by using the favorable conditions of micro-oxygen,and sufficient material and energy. However,the limitation of symbiotic nitrogen fixation is host specificity,that is,symbiotic nitrogen-fixing bacteria are difficult to complete infection and nitrogen fixation on most economic crops. Therefore,an important challenge for nitrogen-fixing synthetic biology is how to break through the host specificity of nitrogen-fixing bacteria,and achieve symbiotic or independent nitrogen fixation on main economic crops. In order to solve this difficult problem,domestic and abroad researchers have made fine research progress through hard exploration. This review will give a brief overview of some major advances and issues in the biology of nitrogen-fixing synthesis.

Key words: biological nitrogen fixation, synthetic biology, organelle, nitrogenase, nodulation factors

LUO Li. Synthetic Biology Research of Plant Nitrogen-fixation Organelle[J]. Biotechnology Bulletin, 2019, 35(10): 1-6.

References

[1] Batista MB, Dixon R.Manipulating nitrogen regulation in diazotrophic bacteria for agronomic benefit[J]. Biochemical Society Transactions, 2019, 47:603-614.
[2] Ro DK, Paradise EM, Ouellet M, et al.Production of the antimalarial drug precursor artemisinic acid in engineered yeast[J]. Nature, 2006, 440:940-943.
[3] Fredens J, Wang K, de la Torre D, et al. Total synthesis of Escherichia coli with a recoded genome[J]. Nature, 2019, 569:514-518.
[4] Shao Y, Lu N, Wu Z, et al.Creating a functional single-chromosome yeast[J]. Nature, 2018, 560(7718):331-335.
[5] Shen Y, Wang Y, Chen T, et al. Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome[J]. Science, 2017, 355:eaaf4791.
[6] Gibson DG, Benders GA, Andrews-Pfannkoch C, et al.Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome[J]. Science, 2008, 319:1215-20.
[7] Dixon RA, Postgate JR.Genetic transfer of nitrogen fixation from Klebsiella pneumoniae to Escherichia coli[J]. Nature, 1972, 237(5350):102-103.
[8] Liu D, Liberton M, Yu J, et al.Engineering nitrogen fixation activity in an oxygenic phototroph[J]. MBio, 2018, 9(3):e01029-18.
[9] Zhan Y, Yan Y, Deng Z, et al.The novel regulatory ncRNA, NfiS, optimizes nitrogen fixation via base pairing with the nitrogenase gene nifK mRNA in Pseudomonas stutzeri A1501[J]. Proc Natl Acad Sci USA, 2016, 113(30):E4348-E4356.
[10] Zhu JB, Li ZG, Wang LW, et al.Temperature sensitivity of a nifA-like gene in Enterobacter cloacae[J]. J Bacteriol, 1986, 166(1):357-359.
[11] 戴小密, 刘彦杰, 叶小梅, 等. 接种大豆根瘤菌(Sinorhizobium fredii)遗传工程菌株LMG101对大豆的增产效应[J]. 中国农业科学, 2003, 36(1):66-70.
[12] Temme K, Zhao D, Voigt CA.Refactoring the nitrogen fixation gene cluster from Klebsiella oxytoca[J]. Proc Natl Acad Sci USA, 2012, 109(18):7085-7090.
[13] Burén S, Rubio LM.State of the art in eukaryotic nitrogenase engineering[J]. FEMS Microbiology Letters, 2018, 365(2). doi:10. 1093/femsle/fnx274.
[14] Geddes BA, Ryu MH, Mus F, et al.Use of plant colonizing bacteria as chassis for transfer of N₂-fixation to cereals[J]. Curr Opin Biotechnol, 2015, 32:216-222.
[15] Curatti L, Rubio LM.Challenges to develop nitrogen-fixing cereals by direct nif-gene transfer[J]. Plant Science, 2014, 225:130-137.
[16] Cheng Q, Day A, Dowson-Day M, et al.The Klebsiella pneumoniae nitrogenase Fe protein gene(nifH)functionally substitutes for the chlLgene in Chlamydomonas reinhardtii[J]. Biochemical and Biophysical Research Communications, 2005, 329(3):966-975.
[17] Ivleva NB, Groat J, Staub JM, et al.Expression of active subunit of nitrogenase via integration into plant organelle genome[J]. PLoS One, 2016, 11(8):e0160951.
[18] López-Torrejón G, Jiménez-Vicente E, Buesa JM, et al.Expression of a functional oxygen-labile nitrogenase component in the mitochondrial matrix of aerobically grown yeast[J]. Nat Commun, 2016, 7:11426.
[19] Burén S, Young EM, Sweeny EA, et al.Formation of nitrogenase NifDK tetramers in the mitochondria of Saccharomyces cerevisiae[J]. ACS Synth Biol, 2017, 6(6):1043-1055.
[20] Yang J, Xie X, Yang M, et al.Modular electron-transport chains from eukaryotic organelles function to support nitrogenase activity[J]. Proc Natl Acad Sci USA, 2017, 114(12):E2460-E2465.
[21] Wang L, Zhang L, Liu Z, et al.A minimal nitrogen fixation gene cluster from Paenibacillus sp. WLY78 enables expression of active nitrogenase in Escherichia coli[J]. PLoS Genet, 2013, 9(10):e1003865.
[22] Li XX, Liu Q, Liu XM, et al.Using synthetic biology to increase nitrogenase activity[J]. Microb Cell Fact, 2016, 15:43.
[23] Wang X, Yang JG, Chen L, et al.Using synthetic biology to distinguish and overcome regulatory and functional barriers related to nitrogen fixation[J]. PLoS One, 2013, 8(7):e68677.
[24] Yang J, Xie X, Wang X, et al.Reconstruction and minimal gene requirements for the alternative iron-only nitrogenase in Escherichia coli[J]. Proc Natl Acad Sci USA, 2014, 111(35):E3718-E3725.
[25] Yang J, Xie X, Xiang X, et al.Polyprotein strategy for stoichiometric assembly of nitrogen fixation components for synthetic biology[J]. Proc Natl Acad Sci USA, 2018, 115(32):E8509-E8517.
[26] Marchetti M, Capela D, Glew M, et al.Experimental evolution of a plant pathogen into a legume symbiont[J]. PLoS Biol, 2010, 8(1):e1000280.
[27] Guan SH, Gris C, Cruveiller S, et al.Experimental evolution of nodule intracellular infection in legume symbionts[J]. The ISME Journal, 2013, 7(7):1367-1377.
[28] Dai WJ, Zeng Y, Xie ZP, Staehelin C.Symbiosis-promoting and deleterious effects of NopT, a novel type 3 effector of Rhizobium sp. strain NGR234[J]. J Bacteriol, 2008, 190(14):5101-5110.
[29] Okazaki S, Kaneko T, Sato S, Saeki K.Hijacking of leguminous nodulation signaling by the rhizobial type III secretion system[J]. Proc Natl Acad Sci USA, 2013, 110(42):17131-17136.
[30] Ling J, Wang H, Wu P, et al.Plant nodulation inducers enhance horizontal gene transfer of Azorhizobium caulinodans symbiosis island[J]. PNAS, 2016, 113(48):13875-13880.
[31] Rogers C, Oldroyd GE.Synthetic biology approaches to engineering the nitrogen symbiosis in cereal[J]. J Exp Bot, 2014, 65(8):1939-1946.
[32] Mus F, Crook MB, Garcia K, et al.Symbiotic nitrogen fixation and the challenges to its extension to nonlegumes[J]. Appl Environ Microbiol, 2016, 82(13):3698-3710.
[33] 李文清, 曹文刚, 李豪, 等. 百脉根4个共生基因在水稻中的共表达及其对水稻转录组的影响[J]. 华中农业大学学报, 2017, 4:55-61.