Research Progress on Fluorescent Copper Nanoparticles Mediated Biosensors

doi:10.13560/j.cnki.biotech.bull.1985.2018-0524

Abstract

Abstract: Fluorescent copper nanoparticles（CuNCs）are nanometer-sized copper crystals generated by using deoxyribonucleic acid（DNA）as a template and divalent copper ions（Cu²⁺）and ascorbic acid as reactants,and they are fluorescent and can be used to be output signal in biosensors. The generation of CuNCs is fast,simple,and safe. Therefore,many researches on the principles and applications of CuNCs have emerged in recent years. Here CuNCs mediated biosensors are classified in terms of mediators supporting the sensor operation and signal output mode. A detailed description of the principles of various sensors and comparison of the advantages and disadvantages of the biosensors are included,for the purpose of understanding the feasibility and versatility of（CuNCs）mediated biosensors after reading the development and directions of these biosensors. Finally,this review also discusses the shortcomings in applying the CuNCs biosensors and looks into its future development trend. This is aimed to inspire future research and development in this area and thus to develop copper nanoparticles mediated biosensors into more practical and convenient biosensing tool.

Key words: fluorescence, copper, nanocluster, biosensor, functional nucleic acid

LIU Xing-yu, LI Chun-hui, TIAN Jing-jing, SHAO Xiang-Li, LUO Yun-bo, XU Wen-tao. Research Progress on Fluorescent Copper Nanoparticles Mediated Biosensors[J]. Biotechnology Bulletin, 2019, 35(1): 170-186.

References

[1] Rotaru A, Dutta S, Jentzsch E, et al.Selective dsDNA-templated formation of copper nanoparticles in solution[J]. Angewandte Chemie, 2010, 49(33):5665-5667.
[2] Song QW, Shi Y, He DC, et al.Sequence-dependent dsDNA-templated formation of fluorescent copper nanoparticles[J]. Chemistry, 2015, 21(6):2417-2422.
[3] Qing ZH, He XX, He DM, et al.Poly(thymine)-templated selective formation of fluorescent copper nanoparticles[J]. Angewandte Chemie, 2013, 52(37):9719-9722.
[4] Qing ZH, Qing TP, Mao ZG, et al.dsDNA-templated fluorescent copper nanoparticles:poly(AT-TA)-dependent formation[J]. RSC Advances, 2014, 4(105):61092-61095.
[5] Chen JH, Liu J, Fang ZY, et al.Random dsDNA-templated formation of copper nanoparticles as novel fluorescence probes for label-free lead ions detection[J]. Chem Comm, 2012, 48(7):1057-1059.
[6] Qing ZH, Qing TP, Mao ZG, et al.dsDNA-specific fluorescent copper nanoparticles as a‘green’nano-dye for polymerization-mediated biochemical analysis[J]. Chem Comm, 2014, 50(84):12746-12748.
[7] Zhang L, Cai QY, Li J, et al.A label-free method for detecting biothiols based on poly(thymine)-templated copper nanoparticles[J]. Biosensors and Bioelectronics, 2015, 69:77-82.
[8] Han BY, Xiang RC, Hou XF, et al.One-step rapid synthesis of single thymine-templated fluorescent copper nanoclusters for “turn on” detection of Mn²+[J]. Analytical Methods, 2017, 9(17):2590-2595.
[9] Liu J, Chen JH, Fang ZY, et al.A simple and sensitive sensor for rapid detection of sulfide anions using DNA-templated copper nanoparticles as fluorescent probes[J]. Analyst, 2012, 137(23):5502-5505.
[10] Chen ZZ, Niu YX, Cheng GY, et al.Fast, highly sensitive and selective assay of iodide ions with single-stranded DNA-templated copper nanoparticles as a fluorescent probe for its application in Kunming mice samples[J]. Analyst, 2017, 142(15):2781-2785.
[11] Zhao J, Hu SS, Cao Y, et al.Electrochemical detection of protein based on hybridization chain re-action-assisted formation of copper nanoparticles[J]. Biosensors and Bioelectronics, 2015, 66:327-331.
[12] Song CX, Yang XH, Wang KM, et al.label-free and non-enzymatic detection of DNA based on hybridization chain reaction amplification and dsDNA-templated copper[J]. Analytica Chimica Acta, 2014, 827(3):74-79.
[13] Xu FZ, Shi H, He XX, et al.Concatemeric dsDNA-templated copper nanoparticles strategy with improved sensitivity and stability based on rolling circle replication and its application in microRNA detection[J]. Anal Chem, 2014, 86(14):6976-6982.
[14] Wang XP, Yin BC, Ye BC.A novel fluorescence probe of dsDNA-templated copper nanoclusters for quantitative detection of microsRNAs[J]. RSC Advances, 2013, 3(23):8633-8636.
[15] Hu R, Liu YR, Kong RM, et al.Double-strand DNA-templated formation of copper nanoparticles as fluorescent probe for Label free nuclease enzyme detection[J]. Biosensors and Bioelectronics, 2013, 42(4):31-35.
[16] Zhang H, Lin ZH, Su XG. label-free detection of exonuclease III by using dsDNA-templated copper nanoparticles as fluorescent probe[J]. Talanta, 2015, 131:59-63.
[17] Zhang ll, Zhao JJ, Zhang H, et al. Double strand DNA-templated copper nanoparticle as a novel fluorescence indicator for label-free detection of polynucleotide kinase activity[J]. Biosensors and Bioelectronics, 2013, 44(18):6-9.
[18] Zhao HZ, Dong JJ, Zhou FL, et al.One facile fluorescence strategy for sensitive detection of endonuclease activity using DNA-templated copper nanoclusters as signal indicators[J]. Sensors and Actuators B, 2017, 238:828-833.
[19] Qing TP, He XX, He DG, et al.Dumbbell DNA-templated CuNCs as a nano-fluorescent probe for detection of enzymes involved in ligase-mediated DNA repair[J]. Biosensors and Bioelectronics, 2017, 94:456-463.
[20] Xu FZ, Luo L, Shi H, et al.label-free and sensitive microRNA detection based on a target recycling amplification-integrated superlong poly(thymine)-hosted copper nanoparticle strategy[J]. Analytica Chimica Acta, 2018, 1010:54-61.
[21] Qing ZH, Qing TP, Mao ZG, et al.dsDNA-specific fluorescent copper nanoparticles as a‘green’nano-dye for polymerization-mediated biochemical analysis[J]. Chem Comm, 2014, 50:12746-12748.
[22] Ou LJ, Li XY, Li LJ, et al.A sensitive assay for trypsin using poly(thymine)-templated copper nanoparticles as fluorescent probes[J]. Analyst, 2015, 140(6):1871-1875.
[23] Wang L, Shi FP, Li Y.An ultra-sensitive and label-free fluorescent probe for trypsin and inhibitor based on DNA hosted Cu nanoclusters[J]. Sensors and Actuators B:Chemical, 2016, 222:945-951.
[24] Zhang ll, Zhao JJ, Duan M, et al. Inhibition of dsDNA-templated copper nanoparticles by pyrophosphate as a label-free fluorescent strategy for alkaline phosphatase assay[J]. Anal Chem, 2013, 85(8):3797-3801.
[25] Li JY, Si L, Bao JC, et al.Fluorescence regulation of poly(thymine)-templated copper nanoparticles via an enzyme-triggered reaction towards sensitive and selective detection of alkaline phosphatase[J]. Anal Chem, 2017, 89(6):3681-3686.
[26] Chi BZ, Liang RP, Qiu WB, et al.Direct fluorescence detection of microRNA based on enzymatically engineered primer extension poly-thymine(EPEPT)reaction using copper nanoparticles as nano-dye[J]. Biosensors and Bioelectronics, 2107, 87:216-221.
[27] Chen J, Xu Y, Ji XH, et al.Enzymatic polymerization-based formation of fluorescent copper nanoparticles for the nuclease assay[J]. Sensors and Actuators B, 2017, 239:262-269.
[28] Wang HB, Zhang HD, Chen Y, et al.A fluorescent biosensor for protein detection based on poly(thymine)- templated copper nanoparticles and terminal protection of small molecule-linked DNA[J]. Biosensors and Bioelectronics, 2015, 74(2):581-586.
[29] Sheng FF, Zhang XJ, Wang GF.Novel ultrasensitive homogeneous electrochemical aptasensor based on dsDNA-templated copper nanoparticles for the detection of ractopamine[J]. Journal of Materials Chemistry B, 2017, 5(1):53-61.
[30] Wang HB, Zhang HD, Chen Y, et al.A label-free and ultrasensitive fluorescent sensor for dopamine detection based on double-stranded DNA templated copper manoparticles[J]. Sensors and Actuators B:Chemical, 2015, 220:146-153.
[31] Zhu HW, Dai WX, Yu XD, et al.Poly thymine stabilized copper nanoclusters as a fluorescence probe for melamine sensing[J]. Talanta, 2015, 144:642-647.
[32] Wang ZX, Han P, Mao XX, et al.Sensitive detection of glutathione by using DNA-templated copper nanoparticles as electrochemical reporters[J]. Sensors and Actuators B, 2017, 238:325-330.
[33] Yang LZ, Wang YJ, Li BX, et al.High-throughput identification of telomere-binding ligands based on the fluorescence regulation of DNA-copper nanoparticles[J]. Biosensors and Bioelectronics, 2017, 87:915-917.
[34] Sun FF, You Y, Liu J, et al.DNA three-way junction for differentiation of single nucleotide polymorphisms with fluorescent copper nanoparticles[J]. Chemistry-A European Journal, 2017, 23(29):6979-6982.
[35] Borghei Y, Hosseini M, Ganjali MR, et al.Label-free fluorescent detection of microRNA-155 based on synthesis of hairpin DNA-templated copper nanoclusters by etching(top-down approach)[J]. Sensors and Actuators B, 2017, 248:133-139.
[36] Qing ZH, Zhu LX, Yang S, et al.In situ formation of fluorescent copper nanoparticles for ultrafast zero-background Cu²+ detection and its toxicides screening[J]. Biosensors and Bioelectronics, 2016, 78:471-476.
[37] Zhou ZX, Du Y, Dong SJ.Double-strand DNA-templated formation of copper nanoparticles as fluorescent probe for label-free aptamer sensor[J]. Anal Chem, 2011, 83(13):5122-5127.
[38] Song QW, Wang RH, Sun FF, et al.A nuclease-assisted label-free aptasensor for fluorescence turn-on detection of ATP based on the in situ formation of copper nanoparticles[J]. Biosensors and Bioelectronics, 2017, 87:760-763.
[39] Cao JP, Wang W, Bo B, et al.A dual-signal strategy for the solid detection of both small molecules and proteins based on magnetic separation and highly fluorescent copper nanoclusters[J]. Biosensors and Bioelectronics, 2017, 90:534-541.
[40] Mao ZG, Qing ZH, Qing TP, et al.Poly(thymine)-templated copper nanoparticles as a fluorescent indicator for hydrogen peroxide and oxidase-based biosensing[J]. Anal Chem, 2015, 87(14):7454-7460.
[41] Chen J, Ji XH, He ZK.Smart composite reagent composed of double-stranded DNA- templated copper nanoparticle and SYBR green I for hydrogen peroxide related biosensing[J]. Anal Chem, 2017, 89(7):3988-3995.