Mutant Construction and Functional Validation of NHX1 in Saccharomyces cerevisiae BJ3505

doi:10.13560/j.cnki.biotech.bull.1985.2018-0561

Abstract

Abstract: A Saccharomyces cerevisiae mutant with deficient NHX1 gene was obtained for verifying its function in S. cerevisiae BJ3505's resistance to stress and vacuole fusion. The BJ3505Δnhx1,the mutant of BJ3505 with inactivated NHX1,was cloned via homologous recombination. Then the complementary strain BJ3505Δnhx1-C was constructed to validate the function of NHX1 in stress tolerance and vacuole fusion. As results,mutant BJ3505Δnhx1 had a much lower ability in stress tolerance and an increase in cells harboring multiple vacuoles was observed,compared with the BJ3505Δnhx1-C and the wild one. Conclusively,the NHX1gene-deleted mutant strain was constructed successfully by homologous recombination. As a crucial ion antiporter,NHX1 plays an important role in the stress resistance and vacuole fusion of S. cerevisiae BJ3505.

Key words: Saccharomyces cerevisiae, NHX1, adversity stress, vacuole fusion

WANG Li-guang, CHEN Jun, YE Chun-lei, LUO Jun-jie. Mutant Construction and Functional Validation of NHX1 in Saccharomyces cerevisiae BJ3505[J]. Biotechnology Bulletin, 2018, 34(12): 152-158.

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