Development Progress of Digital PCR in the Precise Detection of Functional Nucleic Acid

doi:10.13560/j.cnki.biotech.bull.1985.2018-0568

Abstract

Abstract: Digital PCR,as a quantitative analysis technology,has developed rapidly in recent years. The analysis of results from it doesn’t depend on the cycle of threshold（Ct）of the amplification curve and is also not affected by the amplification efficiency,it has a very promising accuracy and reproducibility,and the absolute quantitative analysis can be achieved. Digital PCR has shown great advantages and application prospects in the detection and identification of functional nucleic acids. Based on the introduction of the basic principles and quantitative methods of digital PCR technology,this paper reviews the main application fields and forecasts the research prospect of digital PCR in the detection of functional nucleic acids.

Key words: digital PCR, functional nucleic acid, precise detection

LIU Xiao, ZHU Peng-yu, WANG Yao, ZHU Shui-fang, FU Wei. Development Progress of Digital PCR in the Precise Detection of Functional Nucleic Acid[J]. Biotechnology Bulletin, 2018, 34(9): 149-162.

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