Research Progress on 10-23 DNAzyme Mediated Biosensors

doi:10.13560/j.cnki.biotech.bull.1985.2017-1101

Abstract

Abstract: In the past two decades,many DNA-based biosensors emerged and developed rapidly. While the main role of DNA is genetic information container,its folded space structure is functional as well. The theories and applications of functional nucleic acids have been involved in many critical fields such as biosensor,bioimaging and medication. As one of them,10-23 DNAzyme of a functional nucleic acid was obtained using in vitro selection technology. It can be made to specifically recognize and cleave any targeted RNA substrate at cleavage site of phosphodiester bond between pyrimidine and purine at the presence of Mg²⁺. Because of its special identification and cleavage ability,10-23 DNAzyme mediated disease treatment has been applied widely;also 10-23 DNAzyme mediated biosensors have been established. In this paper,we present the structure,property,action mechanism,and modification of 10-23 DNAzyme;meanwhile,we summarize the establishment and application of 10-23 DNAzyme-mediated biosensors,aiming at providing theoretical basis to guide people to develop new 10-23 DNAzyme-mediated biosensors.

Key words: 10-23 DNAzyme, functional nucleic acid, biosensor, bioimaging, Mg²⁺

LI Kai, LUO Yun-bo, XU Wen-tao. Research Progress on 10-23 DNAzyme Mediated Biosensors[J]. Biotechnology Bulletin, 2019, 35(1): 140-150.

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