Transcriptome Sequencing and Analysis of Okra Fruit

doi:10.13560/j.cnki.biotech.bull.1985.2017-0765

Abstract

Abstract: In order to study the expressions of functional genes in the transcriptome of okra fruit，RNA-Seq technology was used to sequence 3 samples of okra fruit. Sequencing results showed that three samples received a total of 77 476 Unigene sequences after assembly，and there were annotations in 4 databases for 61 891 Unigene sequences，accounted for 79.88%. Based on KEGG metabolic pathway database，13 336 Unigene sequences of okra fruit may be classified into 128 metabolic pathways. And 3830 SSR（Simple Sequence Repeats）loci were discovered in okra fruit transcriptome，distributed in 3569 Unigenes，accounting for 4.61% of the transcriptome sequences.

Key words: Abelmoschus esculentus, fruit, RNA-Seq technology, SSR marker

LI He-ping, YAO Yun-fa, LIAN Dong-mei, LAI Zheng-feng, HONG Jian-ji. Transcriptome Sequencing and Analysis of Okra Fruit[J]. Biotechnology Bulletin, 2018, 34(3): 121-127.

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