Molecular Marker-assisted Selection in Melon Sex Expression

doi:10.13560/j.cnki.biotech.bull.1985.2018-0895

Abstract

Abstract: The objective of this study is to identify the sex type of melon via using A and G genes,and to provide a method for molecular marker-assisted breeding of other traits in melon. Based on the structures of the published CmACS-7（A）gene and WIP1+Gyno-hAT（G）gene in melon,PCR primers were designed,by which the detection of 47 recombinant inbred lines families and 14 melon materials was verified. Results indicated that PCR products from primer CmACS-7 was enzymatically digested at 383 bp,those with the genotype AA was not able to be digested by enzyme,and those digested by enzyme were in genotype aa,moreover,endonuclease locus discernible co-dominant genotype. Two primers were designed based on WIP1+Gyno-hAT gene structure,G（g）genotype was distinguished between 197 and 184 bp of the amplified bands. In conclusion,the self-designed primers can be used in the molecular marker-assisted selection breeding of monoecious,gynoecious,pure male line,and complete-flower melon at seedling stage.

Key words: melon, sex expression, marker-assisted selection

FAN Lei, DAI Dong-yang, XIONG An-ping, SHENG Yun-yan, YU Ming-zhu, QIN Ying-cong. Molecular Marker-assisted Selection in Melon Sex Expression[J]. Biotechnology Bulletin, 2019, 35(4): 195-200.

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