Abstract: Microbial secondary metabolites are important sources of drugs. When the traditional activity-oriented screening mechanism is stagnant，how to adopt new screening methods to reduce the repeated detection of known compounds and to quickly obtain new compounds with novel structures and special biological activities is the key to drug development. Genomic mining technology has become a research hotspot at home and abroad because it may control the directed expression of genes encoding secondary metabolites，combine scientific theories with industrial production and guide them，effectively reduce the development cost and cycle，and contribute to the final market application. In this paper，the research direction of genome mining technology and the technical means of activating silenced gene expression are summarized，the important status of fungi in genome mining and the research achievements in recent years are also described，and the broad prospect of fungi in the field of medicine is highlighted.

Key words: secondary metabolites, genome mining, activation of silenced gene