Physiological Mechanism of Exogenous Ethylene and Sulfur in Alleviating Cadmium Stress in Portulaca oleracea

doi:10.13560/j.cnki.biotech.bull.1985.2019-0303

Abstract

Abstract: To uncover the physiological mechanism of exogenous ethylene and sulfur（S）in response to cadmium（Cd）stress,the effects of ethephon（ethylene donor）on the oxidative stress,S assimilation,glucose content,ethylene biosynthesis,and photosynthesis in leaf of P. oleracea were detected. The results revealed that exogenous ethylene and S-treatment decreased the Cd content in the leave and root of P. oleracea under Cd stress,also decreased H₂O₂accumulation and malondialdehyde（MDA）content via enhancing the activities of ATP sulfase（ATP-S）and glutathione reductase（GR）as well as increasing the content of reduced glutathion（GSH）in P. oleracea leaves. Moreover,the application of S or ethylene resulted in the glucose content reducing and Rubisco enzyme activity and photosynthesis increasing via increasing the activity of 1-aminocyclopropane carboxylic acid enzyme（ACS）and ethylene content in leaves. Simultaneous addition of exogenous ethylene and S enhanced above promoting and inhibitory effects,but the application of ethylene action inhibitor norbornadiene（NBD）resulted in the reverse effects of above indexes. The results suggest that exogenous S and ethylene alleviates the Cd- induced oxidative stress and glucose-mediated photosynthesis inhibition in P. oleracea through decreasing Cd absorption,enhancing internal ethylene signaling pathways,promoting GSH production,and decreasing glucose content.

Key words: cadmium stress, ethylene, sulfur, Portulaca oleracea L., physiological characteristic

WANG Zhu-cheng, LIU Hui, LI Rong-hua, CHEN Xin, LI Xin, LU Zhi-yuan. Physiological Mechanism of Exogenous Ethylene and Sulfur in Alleviating Cadmium Stress in Portulaca oleracea[J]. Biotechnology Bulletin, 2019, 35(10): 71-79.

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