Research Progress on RNA-cleaving DNAzyme in the Detection of Pathogens

doi:10.13560/j.cnki.biotech.bull.1985.2019-0568

Abstract

Abstract: DNA is an important carrier of genetic information,and its folding property of spatial conformation makes it have many functions. Using a RNA-cleaving DNAzyme to recognize a specific single-stranded DNA molecule and to cleave one of the single strands,a signal output sensor can be constructed,which converts the specific recognition process into the signal outputs of gel electrophoresis character,fluorescence,and colorimetric,and it can be combined with amplification reaction to achieve signal amplified. RNA-cleaving DNAzymes are obtained by in vitro screening techniques that specifically bind to target substances（such as small molecules,proteins,and even whole cells）. Due to its advantages of convenient preparation,easy modification and fine stability,RNA-cleaving DNAzymes are used to construct biosensors to detect pathogenic microorganisms,for example in-situ detection and even in-vivo detection in medical treatment. In the future,it will be combined with mature detection,for instance,equipment blood glucose meter,cross-flow chromatography strips for microbiological testing. Furthermore,it can be used in important areas such as biosensing,food safety,and medical care. Here we review the application of RNA-cleaving DNAzymes in microbial detection. In addition,we discuss the mechanism,products,targets and characterization of RNA-cleaving DNAzymes in microbial detection,as well as the significance and problems of RNA-cleaving DNAzymes in the actual detection of microorganisms. Finally,we prospect the further application of this technology,aiming at the better development of nucleic acid cleavage enzymes in microbial detection.

Key words: RNA-cleaving DNAzymes, microorganism, biosensing, signal amplification, detection

WANG Xin, ZHU Long-jiao, XU Wen-tao, ZHAI Chen, WANG Shu-ya, HUANG Wei-xia. Research Progress on RNA-cleaving DNAzyme in the Detection of Pathogens[J]. Biotechnology Bulletin, 2020, 36(1): 182-192.

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