Abstract: It aims to explore the effects of tobacco extract on the lifespan and aging of fruit flies and the possible molecular mechanisms. The young W¹¹¹⁸ male fruit flies within 12 h of emergence were cultured in medium supplemented with different concentrations of tobacco extract,and the death of fruit flies was counted daily. The total RNA was extracted from control group and the highest concentration treatment group at every 7 d,and the expressions of life-related genes,such as catalase（CAT）,superoxide dismutase（SOD）,deubiquitinating enzyme（Rpn11）,and deacetylase（Sirt6）,were detected by real-time PCR. The result showed that the effect of tobacco significantly shortened the lifespan of male Drosophila melanogaster,which gradually increased with the increase of tobacco extract concentration. The antioxidant genes of the fruit flies were significantly up-regulated under the influence of tobacco,and the changes in the expressions of ubiquitination regulatory gene and deacetylation gene significantly varied. This indicates that tobacco adversely affects the lifespan of fruit flies,which may lead to premature senescence of fruit flies by causing oxidative stress.

Key words: tobacco, fruit fly, aging, expression regulation, oxidative stress