Effects of Recombinant IL-2 on Blood Biochemical Parameters and Immune Indexes of Takifugu rubripes

doi:10.13560/j.cnki.biotech.bull.1985.2019-0916

Abstract

Abstract: In order to study the effects of recombinant interleukin-2（r IL-2）on blood biochemical parameters and immune indexes of Takifugu rubripes,the T. rubripes with a body weight of（205.9±30.5）g was selected. These T. rubripes were used for the immune response assay of recombinant interleukin 2.The r IL-2 immunization dose was designed to be 2.5,5,7.5,and 10 μg,and blood was collected at 4 h,8 h,and 12 h after immunization. The results showed that the content of urea nitrogen in T. rubripes serum after r IL-2 immunization was significantly higher than that in other groups at the 8h time point（P<0.05）,and the triglyceride content at 4 h time point tended to increase with the rise of immunization dosage. The activity of aspartate aminotransferase at 8 h was higher than that at other time points（P<0.05）. The activity of catalase in 2.5 and 5μg dosage group was significantly higher than that of other groups at 4 h（P<0.05）. It also had a significant effect on the content of immunoglobulin. This study found that the injection of recombinant IL-2 caused changes in blood biochemical indicators and immune indexes,especially the changes of superoxide dismutase and aspartate aminotransferase（P<0.05）,which may promote the immune response of T. rubripes to a certain extent.

Key words: Takifugu rubripes, interleukin-2, blood biochemical index

BAO Ming-xiu, ZANG Lin, ZHANG Hong-bin, LIU Fu-jun, JING Di, WANG Xiu-li. Effects of Recombinant IL-2 on Blood Biochemical Parameters and Immune Indexes of Takifugu rubripes[J]. Biotechnology Bulletin, 2020, 36(3): 177-182.

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