Research Progress of Biosensing Strategy Based on CRISPR/Cas System

doi:10.13560/j.cnki.biotech.bull.1985.2019-0932

Abstract

Abstract: CRISPR/Cas9 system is the third generation of genome editing tools after zinc finger endonuclease and transcription activator-like effector nuclease. Because of its ability to cut double-stranded DNA,CRISPR/Cas9 system is widely used in gene editing and biosensing. With the “collateral cleavage” activity of Cas12a（Cpf1）and Cas13a（C2c2）proteins discovered,the application of CRISPR / CAS system in biosensor has been expanded. In recent years,researchers have developed a series of fast,ultrasensitive and highly specific biosensor for molecular detection,such as SHERLOCK and DETECTR. This review mainly reports the research progress of new biosensors based on CRISPR/CAS system,and prospects its future development.

Key words: CRISPR/Cas system, isothermal amplification, biosensing, ultrasensitive detection, single base specificity

HU Xiao-lin, WANG Liang-ting, GU Wei, LUO Yang. Research Progress of Biosensing Strategy Based on CRISPR/Cas System[J]. Biotechnology Bulletin, 2020, 36(3): 69-77.

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