Identification and Antifungal Activity Determination of an Endophytic Fungus from the Twigs of Vaccinium dunalianum

doi:10.13560/j.cnki.biotech.bull.1985.2020-0200

Abstract

Abstract:

An endophytic fungal strain Z-78 isolated from the twigs of Vaccinium dunalianum Wight was identified as Pestalotiopsis cocculi（Guba）G.C. Zhao & N. Li by morphological classification and molecular biological study. The plate confrontation method was used to determine its antifungal activities against eight plant pathogens including Botrytis cinerea,Fusarium solani,Fusarium graminearum,Fusarium oxysporum,Alternaria brassicicola,Gloeophyllum trabeum,Poria placenta and Coriolus versicolor,and the growth rate method was applied to conduct the acid-base stability test of the fermentation broth inhibiting the activity of A. brassicicola. The results showed that Z-78 strain presented strong inhibitory effects on all of the eight plant pathogens,while the activity against A. brassicicola remained stable under different pH conditions of fermentation broth.

Key words: Vaccinium dunalianum, twig, endophytic fungi, Pestalotiopsis cocculi, antifungal activity

YAN Dong, ZENG Wei-lin, LUO Xu-lu, CHEN Xiao-xue, LIU Hui-min, ZHAO Ping. Identification and Antifungal Activity Determination of an Endophytic Fungus from the Twigs of Vaccinium dunalianum[J]. Biotechnology Bulletin, 2020, 36(11): 30-38.

Figures/Tables 6

References 47

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病原真菌	生长直径/mm	抑菌率/%	抑菌带宽度/mm	抑菌指数
密粘褶菌	25±1.8	72.22	0.00	+++
绵腐卧孔菌	25±1.3	70.39	3.00	+++
尖孢镰孢	32±2.4	61.76	0.00	+++
禾谷镰孢	30±2.5	61.11	0.00	+++
芸苔链格孢	35±2.0	61.00	3.00	+++
采绒革盖菌	35±1.4	57.84	0.00	+++
腐皮镰孢	41±1.9	52.35	0.00	+++
灰葡萄孢	42±2.8	50.00	0.00	+++

病原真菌	生长直径/mm	抑菌率/%	抑菌带宽度/mm	抑菌指数
密粘褶菌	25±1.8	72.22	0.00	+++
绵腐卧孔菌	25±1.3	70.39	3.00	+++
尖孢镰孢	32±2.4	61.76	0.00	+++
禾谷镰孢	30±2.5	61.11	0.00	+++
芸苔链格孢	35±2.0	61.00	3.00	+++
采绒革盖菌	35±1.4	57.84	0.00	+++
腐皮镰孢	41±1.9	52.35	0.00	+++
灰葡萄孢	42±2.8	50.00	0.00	+++

pH	2 d		4 d		6 d		8 d		10 d
pH	净生长半径/mm	抑菌率/%	净生长半径/mm	抑菌率/%	净生长半径/mm	抑菌率/%	净生长半径/mm	抑菌率/%	净生长半径/mm	抑菌率/%
2.0	5.1±0.38	47.9	9.7±0.38	49.6	13.3±0.25	54.4	17.0±0.25	55.7	19.9±0.14	53.1
4.0	5.4±0.14	44.4	9.4±0.29	50.9	13.0±0.43	55.3	16.3±0.14	57.5	18.5±1.15	56.5
6.0	5.5±0.25	43.6	9.2±0.14	52.2	12.8±0.29	55.9	16.1±0.29	58.1	19.2±0.14	54.9
8.0	6.3±0.25	35.9	10.8±0.10	43.9	14.8±0.38	49.0	18.2±0.14	52.7	22.4±0.18	47.4
10.0	5.2±0.14	47.0	8.9±0.14	53.5	12.3±0.29	57.6	15.4±0.14	59.9	18.7±0.14	56.1

pH	2 d		4 d		6 d		8 d		10 d
pH	净生长半径/mm	抑菌率/%	净生长半径/mm	抑菌率/%	净生长半径/mm	抑菌率/%	净生长半径/mm	抑菌率/%	净生长半径/mm	抑菌率/%
2.0	5.1±0.38	47.9	9.7±0.38	49.6	13.3±0.25	54.4	17.0±0.25	55.7	19.9±0.14	53.1
4.0	5.4±0.14	44.4	9.4±0.29	50.9	13.0±0.43	55.3	16.3±0.14	57.5	18.5±1.15	56.5
6.0	5.5±0.25	43.6	9.2±0.14	52.2	12.8±0.29	55.9	16.1±0.29	58.1	19.2±0.14	54.9
8.0	6.3±0.25	35.9	10.8±0.10	43.9	14.8±0.38	49.0	18.2±0.14	52.7	22.4±0.18	47.4
10.0	5.2±0.14	47.0	8.9±0.14	53.5	12.3±0.29	57.6	15.4±0.14	59.9	18.7±0.14	56.1