Isolation,Screening and Identification of Active Endophytic Fungi from Yili Wild Walnut

doi:10.13560/j.cnki.biotech.bull.1985.2019-0443

Abstract

Abstract: The objective of this work is to screen the endophytic fungi with strongly antibacterial and antioxidant activities from Yili wild walnut and then identify them. We used tissue separation method to isolate the endophytic fungi from wild walnut,and the plate confrontation to conduct the experiment on their antibacterial activities as well as their DPPH radical scavenging capacity. Finally,the strains were identified. As results,（1）49 strains of endophytic fungi were isolated. The most isolates came from the stem,then from the roots,and none from the kernels.（2）Strain YHT-4 and YHT-6 presented significant antibacterial effects and the inhibitory index was > 10,and YHT-4 inhibited pathogenic fungus with diameter > 1.5 cm.（3）The strain YHT-4 demonstrated a stronger ability to scavenge DPPH free radicals which was equivalent to Vc.（4）The strain YHT-4 was molecularly identified as Fusarium tricinctum based on morphology and molecular science. In conclusion,the isolated strain F. tricinctum sp. YHT-4 had strong antibacterial and anti-oxidant activities,and it can he used as potential sources for drug development in further study.

Key words: Yili wild walnut, endophytic fungi, antibacterial activity, antioxidant

RANG Feng-ju, REN Yan-li, ZHANG Wei, OUYANG Yan. Isolation,Screening and Identification of Active Endophytic Fungi from Yili Wild Walnut[J]. Biotechnology Bulletin, 2019, 35(9): 218-223.

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