Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (8): 162-175.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0273

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Analysis of Differential Metabolites and Bacterial Community Structure in Soils of a Pineapple Orchard in Different Continuous-cropping Years

LIU Chuan-he1(), HE Han1, HE Xiu-gu2, LIU Kai1, SHAO Xue-hua1, LAI Duo1, KUANG Shi-zi1, XIAO Wei-qiang1   

  1. 1. Institute of Fruit Tree Research,Guangdong Academy of Agricultural Sciences/Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization,Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research,Guangzhou 510640
    2. Guangdong Academy of Agricultural Sciences,Guangzhou 510640
  • Received:2021-03-10 Online:2021-08-26 Published:2021-09-10

Abstract:

In order to investigate the effects of continuous-cropping pineapple on the soil metabolites and bacteria community structure,taking the soil of uncultivated land(T0)on the edge of the orchard as the control,untargeted metabolomics and bacterial 16S high-throughput sequencing were combined to compare and analyze the metabolites of the soils continuous-cropping pineapple for 5(T5)and 15(T15)years respectively as well as differences in bacterial community. The results of metabolites analysis indicated that there were totally 120 and 80 differential metabolites in the soil of T5 and T15 when compared with T0. And 11 significantly different metabolites(VIP>1,P<0.05)were detected between T0-T5 group and T0-T15 group in total,including 4 organic acids,3 carbohydrates,2 amino acid derivatives,2 alcohols. KEGG metabolic pathways analysis suggested that the differential metabolites were mostly enriched in 11 key metabolic pathways,e.g.,biosynthesis of unsaturated fatty acids. The bacterial 16S high-throughput sequencing profiling indicated that the bacteria diversity and abundance of the soil in T5 increased when compared with T0. The bacteria diversity increased while the abundance decreased in T15 soil when compared with T0. And there were significant differences in bacterial community structure between T5 and T15. In the levels of phyla and genus,the relative abundances of soil bacteria in T5 and T15 were significantly higher than that in T0. The proportion of two dominant bacteria communities in the soil of the pineapple orchard,Proteobacteria and Acidobacteriota,increased in T5 but decreased in T15 when compared with T0. CCA analysis showed that ccontinuous-cropping years were in significantly negative correlation with pH. This work would contribute to provide a reference for related research on the impacts of ccontinuous-cropping pineapple on the soil environment and contribute to provide the guidance for improving soil management level of pineapple orchard.

Key words: continuous-cropping pineapple, soil, differential metabolites, bacterial community structure