Strategies for Efficient Exogenous Gene Expression in Transgenic Animals

doi:10.13560/j.cnki.biotech.bull.1985.2019-0989

Abstract

Abstract: The exogenous genes are often silenced in transgenic animals or cells due to a variety of reasons. Currently,there are two main strategies to break this bottleneck：one is to use safe harbors;the second is to optimize cis-acting elements of exogenous genes. Using safe harbors is to insert exogenous genes into the active transcription region via site-specific integration technologies. Optimizing cis-acting elements is to select the appropriate promoters,enhancers,introns,ubiquitous chromatin opening elements（UCOE）,matrix attachment regions（MARs）to construct efficient expression vector. This review summarizes the relevant strategies for improving the expression efficiency of exogenous genes and provides a reference for genetic engineering animal breeding.

Key words: transgenic animals, exogenous genes, safe harbors, gene editing

ZHAO Xu-dong, HUANG Yong-zhi, BI Yan-zhen, DONG Fa-ming. Strategies for Efficient Exogenous Gene Expression in Transgenic Animals[J]. Biotechnology Bulletin, 2020, 36(3): 45-53.

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