Effects of High-dose Tannic Acid on the Intestinal Barrier Function and Gut Microbiota in Mice

doi:10.13560/j.cnki.biotech.bull.1985.2021-0860

Abstract

Abstract:

The objective of this study is to analyze the effects of high-dose tannic acid on the intestinal barrier and gut microbiota in normal-diet mice and high-fat diet-induced obese model mice. H&E staining,RT-qPCR,16S rRNA sequencing and other methods were used for detection and analysis. It was found that the intervention of high-dose tannic acid（400 mg/kg）reduced the body weights and food intakes of the mice,and increased the contents of various intestinal segments in the mice,among which the colon content significantly increased. In addition,high-dose tannic acid damaged the intestinal function and intestinal barrier,such as reduced the number of goblet cell and the length of crypt,and decreased intestinal tight junction protein（ZO-1,Occludin,and Claudin）expression. Moreover,oral administration of high-dose tannic acid changed the diversity of gut microbiota in the colon,and increased the abundance of SCFAs-producing bacteria Alistipes,Ruminococcus and Blautia,as well as obesity negatively related bacteria Alistipes and Oscillibacter. In conclusion,the results showed that the damage of intestinal mucosal barrier caused by high-dose tannic acid affected the digestion and absorption of food in mice,which might be the main potential reason for the rapid weight loss in the mice after the intervention of high-dose tannic acid. On the other hand,the changes of gut microbiota caused by high-dose tannic acid also had an effect on the body weights of the mice.

Key words: tannic acid, colon content, intestinal barrier, gut microbiota

HE Ya-lun, ZENG Li-rong, LIU Xiong, ZHANG Ling, WANG Qiong. Effects of High-dose Tannic Acid on the Intestinal Barrier Function and Gut Microbiota in Mice[J]. Biotechnology Bulletin, 2022, 38(4): 278-287.

Figures/Tables 8

Table 1 Primers’ sequences for RT-qPCR

Gene	Forward primer（5'-3'）	Reverse primer（5'-3'）
ZO 1	GCTTTAGCGAACAGAAGGAGC	TTCATTTTTCCGAGACTTCACCA
Occludin	CACACTTGCTTGGGACAGAG	TAGCCATAGCCTCCATAGCC
GAPDH	TGGCCTTCCGTGTTCCTAC	GAGTTGCTGTTGAAGTCGCA

Fig. 1 Effects of high-dose tannic acid on the body weights of the mice A：Changes in the body weights of the four groups of mice during the experiment. B：Weight gains of mice after the end of the experiment. C：The weekly dietary intake of each group during the experiment. D：The weight of adipose tissue in each group after the end of the experiment. The error lines in the figure all are the mean ± standard error,and *（0.01<P<0.05）,**（0.001<P<0.01）,and ***（P<0.001）,the same below

Fig. 2 Effects of high-dose tannic acid on the body fats of the mice A：H&E staining sections of adipose tissue. B：Fat droplet area

Fig. 3 Effects of high-dose tannic acid on the intestinal structure A：Weights of intestinal tissues of the mice. B：Weights of intestinal tissue contents of the mice. C：H&E staining of colon tissue. D：Number of goblet cells. E：Crypt length. F：Tissue damage score

Fig. 4 Effect of high dose tannic acid on the intestinal barrier A：Changes in intestinal Muc2 mRNA content. B：Changes in the mRNA levels of genes related to intestinal barrier function

Fig. 5 Effects of high dose tannic acid on the intestinal microbial diversity of the obese mice A：Alpha diversity index analysis. B：Beta diversity analysis. C：Venn diagram. D：Heatmap at the phylum level

Fig. 6 Analysis of species composition and inter-group differences at the phylum level A：Community bar plot at the phylum level. B and C：Intergroup difference analysis - multi-group comparison

Fig. 7 Analysis of species composition and inter-group differences at the genus level A：Community bar plot at the genus level. B and C：Intergroup difference analysis - multi-group comparison

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