Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (6): 166-173.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1197

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Characteristics of Endophytic Fungal and Bacterial Community in the Stalks of Sugarcane Cultivars Resistant to Ratoon Stunting Disease

GAO Xiao-ning1,2(), LIU Rui1, WU Zi-lin1, WU Jia-yun1   

  1. 1. Institute of Nanfan & Seed Industry,Guangdong Academy of Sciences,Guangzhou 510316
    2. Zhanjiang Sugarcane Research Center,Guangzhou Sugarcane Industry Research Institute,Zhanjiang 524300
  • Received:2021-09-16 Online:2022-06-26 Published:2022-07-11
  • Contact: GAO Xiao-ning E-mail:gxn336@126.com

Abstract:

By analyzing the structural characteristics of endophytic fungi and bacteria in the stalks of sugarcane varieties resistant to ratoon stunting disease(RSD),the aim is to understand the roles of endophytic fungi and bacteria in the resistance of sugarcane,so as to provide theoretical basis for ecological control of RSD. Using Illumina MiSeq high-throughput sequencing method,the communities,diversity index,and differences of endophytic bacteria and fungi between susceptible variety YT60 and resistant variety HoCP95988 were compared. The Alpha diversity indexes of endophytic fungi and bacteria in the stalks of susceptible cultivar YT60 was higher than that of resistant cultivar HoCP95988,indicating more species composition. The endophytic bacteria mainly distributed in Proteobacteria,while Ascomycota and Basidiomycota were the dominant phyla of endophytic fungi. The species compositions of 34 genera in 4 phyla Planctomycetes,Chloroflexi,Gemmatimonadetes,and Tenericutes between susceptible and resistant varieties were significantly different. Among them,the relative abundance of different species was higher in susceptible variety YT60. The diversity of endophytic fungal and bacterial communities in the stalks of sugarcane varieties resistant to RSD were relatively rich,and there were significant differences in the composition. Meanwhile,the classification information of different species provided clues to explore the biocontrol microbial resources for control RSD.

Key words: sugarcane, ratoon stunting disease, endophytic fungi, endophytic bacteria, diversity index, population composition