Genetic Analysis and Gene Mapping of Sorghum Double-grain Mutant Dgs

doi:10.13560/j.cnki.biotech.bull.1985.2021-1294

Abstract

Abstract:

Grain number per panicle is very important for crop yield. In order to identify and clone the gene controlling grain number per panicle,a double-grain mutant gene of sorghum[Sorghum bicolor（L.）Moench]was located,for laying a foundation for gene cloning and functional analysis and providing evidences for the regulation and analysis of sorghum yield traits. The flower organ phenotypes of wild-type sorghum plant and double-grain mutant were observed by stereoscopy. A F₂ population was constructed from the cross between S. bicolor Jin 5 and the double-grain mutant to analyze the inheritance pattern of the mutant and gene mapping. BSA-seq sequencing and map cloning were used for linkage analysis and gene mapping. The results showed that,compared with wild type,the mutant mainly showed an increase in the number of pistil and stamen,ultimately two kernels in one pair of glumes. Genetic analysis indicated that the double-grain trait was controlled by a pair of dominant genes. Gene mapping showed that the target gene was located between markers Indel2930 and SSR7060 on the chromosome 6 of S. bicolor with physical distance of 404 kb. Dgs is a novel sorghum double-grain mutant gene.

Key words: sorghum, double-grain mutant, flower organ, gene mapping, genetic analycis

ZHOU Shi-chen, YI Zhi-ben, WANG Xin-yi, YANG Xiao-ying, SUN Li-na, LUAN Wei-jiang, LIANG Shan-shan. Genetic Analysis and Gene Mapping of Sorghum Double-grain Mutant Dgs[J]. Biotechnology Bulletin, 2022, 38(7): 171-177.

Figures/Tables 7

References 27

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样品 Sample	初始测序reads数目 Raw reads	过滤后的reads数 Clean reads	过滤后碱基数 Clean base	Q20/%	Q30/%	GC含量 GC content/%
双粒突变体 Dgs	39 005 403	38 995 403	11 679 957 024	97.83	93.6	43.28
晋粱5号 Jin5	38 666 782	38 656 413	11 579 578 194	97.74	93.44	43.86
单粒混池/ Single-grain bulk（S bulk）	148 822 039	148 768 510	44 563 912 284	97.61	93.15	43.25
双粒混池/ Double-grain bulk（D bulk）	156 721 594	156 668 065	46 930 613 822	97.58	93.26	43.67

样品 Sample	初始测序reads数目 Raw reads	过滤后的reads数 Clean reads	过滤后碱基数 Clean base	Q20/%	Q30/%	GC含量 GC content/%
双粒突变体 Dgs	39 005 403	38 995 403	11 679 957 024	97.83	93.6	43.28
晋粱5号 Jin5	38 666 782	38 656 413	11 579 578 194	97.74	93.44	43.86
单粒混池/ Single-grain bulk（S bulk）	148 822 039	148 768 510	44 563 912 284	97.61	93.15	43.25
双粒混池/ Double-grain bulk（D bulk）	156 721 594	156 668 065	46 930 613 822	97.58	93.26	43.67

标记名称 Maker	正向引物 Forward primer（5'-3'）	反向引物 Reverse primer（5'-3'）
Indel2930	CGACGCCGGGATTTCCATTT	TTGGTCCACATCCGAGCAGG
Indel6958	ATTTACGGGTAGCGAGCACT	GGCCGTATCCTCAATCCTGT
SSR7060	GCACACCACCGAGTCAGC	CTCCTCCACCTGCAGCAT
SSR1018	ACCTGTAAATTCCATGCATCGT	CAGAAGGTCAACTGTTTCTCCC
SSR2629	CAAATAACAGCCCCCAAAGG	CTGCTAAGGCGTGCTTTATT
SSR9952	CGCAAATGACGAGTCTTGGT	AGTCGGATCTGATCTAATCGCA
SSR8023	GTGTACGTGACGCGGTGAAA	GTCGTCTCCCTGGTATGTATGG

标记名称 Maker	正向引物 Forward primer（5'-3'）	反向引物 Reverse primer（5'-3'）
Indel2930	CGACGCCGGGATTTCCATTT	TTGGTCCACATCCGAGCAGG
Indel6958	ATTTACGGGTAGCGAGCACT	GGCCGTATCCTCAATCCTGT
SSR7060	GCACACCACCGAGTCAGC	CTCCTCCACCTGCAGCAT
SSR1018	ACCTGTAAATTCCATGCATCGT	CAGAAGGTCAACTGTTTCTCCC
SSR2629	CAAATAACAGCCCCCAAAGG	CTGCTAAGGCGTGCTTTATT
SSR9952	CGCAAATGACGAGTCTTGGT	AGTCGGATCTGATCTAATCGCA
SSR8023	GTGTACGTGACGCGGTGAAA	GTCGTCTCCCTGGTATGTATGG