Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (8): 150-158.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1414

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Transcription of Ethylene Biosynthesis and Signaling Associated Genes in Response to Heterodera glycine Infection

GUO Bin-hui1,2(), SONG Li1()   

  1. 1. Joint International Research Laboratory of Agriculture and Agri-Product Safety,Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou University,Yangzhou 225009
    2. Basic Experimental Teaching Center of Life Science,Yangzhou University,Yangzhou 225009
  • Received:2021-11-12 Online:2022-08-26 Published:2022-09-14
  • Contact: SONG Li E-mail:bhguo@yzu.edu.cn;songli@yzu.edu.cn

Abstract:

Ethylene is an important plant hormone. Its synthesis and signal transduction related genes are involved in the regulation of plant growth,development and defense response. In order to clarify the effects of soybean cyst nematode infection and colonization on ethylene biosynthesis and signal transduction pathway,the expressions of ethylene synthesis gene GmACS and signal transduction gene GmEIN in soybean roots inoculated with and without cyst nematode were compared by fluorescence quantitative PCR. The results showed that compared with susceptible varieties,the ethylene biosynthesis in resistant varieties was significantly regulated,and in which 6 GmACSs genes were significantly induced in PI 548316,while significantly inhibited in PI 437654 and PI 88788. Meanwhile,several GmEINs was slightly up-regulated in 4 resistant varieties,but not significantly regulated in other resistant varieties and two susceptible varieties. The results show that ethylene synthesis in some soybean varieties are significantly regulated by soybean cyst nematode infection and colonization,and the regulated genes can be used as candidates for improving soybean disease resistance breeding.

Key words: soybean, Heterodera glycines, GmACS, GmEIN, gene expression pattern