Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (8): 225-232.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1470
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CHEN Tian-ci1,2(), WU Shao-lan1,2, YANG Guo-hui1,2, JIANG Dan-xia1,2, JIANG Yu-ji1,2, CHEN Bing-zhi1,2()
Received:
2021-11-25
Online:
2022-08-26
Published:
2022-09-14
Contact:
CHEN Bing-zhi
E-mail:chentianci0111@163.com;cbz_2006@163.com
CHEN Tian-ci, WU Shao-lan, YANG Guo-hui, JIANG Dan-xia, JIANG Yu-ji, CHEN Bing-zhi. Effects of Ganoderma resinaceum Alcohol Extract on Sleep and Intestinal Microbiota in Mice[J]. Biotechnology Bulletin, 2022, 38(8): 225-232.
组别Group | 剂量Dose/(mg·kg-1·d-1) | 0 d/g | 7 d/g | 14 d/g | 21 d/g |
---|---|---|---|---|---|
CK | 0 | 29.06±1.19 | 33.67±1.90 | 36.05±1.84 | 37.74±1.95 |
LDG | 250 | 28.86±0.66 | 31.54±1.48 | 33.12±2.06 | 34.21±2.44 |
MDG | 500 | 28.33±0.98 | 31.30±1.75 | 33.23±1.64 | 34.41±2.05 |
HDG | 1 000 | 28.17±1.15 | 31.47±1.24 | 32.59±1.66 | 33.66±1.52 |
Table 1 Effects of GRAE on the body weights of mice
组别Group | 剂量Dose/(mg·kg-1·d-1) | 0 d/g | 7 d/g | 14 d/g | 21 d/g |
---|---|---|---|---|---|
CK | 0 | 29.06±1.19 | 33.67±1.90 | 36.05±1.84 | 37.74±1.95 |
LDG | 250 | 28.86±0.66 | 31.54±1.48 | 33.12±2.06 | 34.21±2.44 |
MDG | 500 | 28.33±0.98 | 31.30±1.75 | 33.23±1.64 | 34.41±2.05 |
HDG | 1 000 | 28.17±1.15 | 31.47±1.24 | 32.59±1.66 | 33.66±1.52 |
组别 Group | 直接睡眠Direct sleep rate/% | 巴比妥钠诱导的睡眠潜伏期 Sleep latency induced by sodium barbiturate/min | 戊巴比妥钠诱导的睡眠时长 Sleep time of pentobarbital sodium-induced/min | 戊巴比妥钠诱导的入睡率 Sleep rate of pentobarbital sodium-induced/% |
---|---|---|---|---|
CK | 0 | 28.88±8.61a | 30.94±1.54a | 10 |
LDG | 0 | 28.51±8.16a | 41.87±0.63a | 20 |
MDG | 0 | 27.70±8.87a | 63.23±2.32b | 40 |
HDG | 0 | 20.53±3.89b | 70.12±1.88c | 80 |
Table 2 Effects of GRAE on the sleep quality of mice
组别 Group | 直接睡眠Direct sleep rate/% | 巴比妥钠诱导的睡眠潜伏期 Sleep latency induced by sodium barbiturate/min | 戊巴比妥钠诱导的睡眠时长 Sleep time of pentobarbital sodium-induced/min | 戊巴比妥钠诱导的入睡率 Sleep rate of pentobarbital sodium-induced/% |
---|---|---|---|---|
CK | 0 | 28.88±8.61a | 30.94±1.54a | 10 |
LDG | 0 | 28.51±8.16a | 41.87±0.63a | 20 |
MDG | 0 | 27.70±8.87a | 63.23±2.32b | 40 |
HDG | 0 | 20.53±3.89b | 70.12±1.88c | 80 |
Sample | Raw_Tags | Raw_Bases | Valid_Tags | Valid_Bases | Valid/% | Q20/% | Q30/% | GC/% |
---|---|---|---|---|---|---|---|---|
CK1 | 84 126 | 42.06 M | 77 501 | 32.07 M | 92.12 | 97.92 | 93.89 | 54.58 |
CK2 | 84 478 | 42.24 M | 77 242 | 32.37 M | 91.43 | 97.39 | 92.52 | 55.27 |
CK3 | 86 751 | 43.38 M | 74 655 | 31.17 M | 86.06 | 94.55 | 85.87 | 54.52 |
CK4 | 86 073 | 43.04 M | 78 882 | 32.96 M | 91.65 | 95.26 | 87.66 | 54.68 |
CK5 | 80 663 | 40.33 M | 71 430 | 29.39 M | 88.55 | 97.39 | 92.63 | 54.17 |
CK6 | 81 281 | 40.64 M | 70 531 | 29.26 M | 86.77 | 97.47 | 92.82 | 54.88 |
HDG1 | 84 427 | 42.21 M | 72 261 | 30.28 M | 85.59 | 97.06 | 91.60 | 54.58 |
HDG2 | 87 332 | 43.67 M | 76 096 | 31.55 M | 87.13 | 97.15 | 91.89 | 54.82 |
HDG3 | 64 752 | 32.38 M | 52 225 | 21.59 M | 80.65 | 87.32 | 73.18 | 55.95 |
HDG4 | 80 293 | 40.15 M | 71 835 | 29.72 M | 89.47 | 96.28 | 89.81 | 54.68 |
HDG5 | 86 806 | 43.40 M | 76 963 | 32.22 M | 88.66 | 97.99 | 93.92 | 55.02 |
HDG6 | 80 087 | 40.04 M | 69 329 | 28.57 M | 86.57 | 98.08 | 94.31 | 54.38 |
Table 3 Effective data statistics
Sample | Raw_Tags | Raw_Bases | Valid_Tags | Valid_Bases | Valid/% | Q20/% | Q30/% | GC/% |
---|---|---|---|---|---|---|---|---|
CK1 | 84 126 | 42.06 M | 77 501 | 32.07 M | 92.12 | 97.92 | 93.89 | 54.58 |
CK2 | 84 478 | 42.24 M | 77 242 | 32.37 M | 91.43 | 97.39 | 92.52 | 55.27 |
CK3 | 86 751 | 43.38 M | 74 655 | 31.17 M | 86.06 | 94.55 | 85.87 | 54.52 |
CK4 | 86 073 | 43.04 M | 78 882 | 32.96 M | 91.65 | 95.26 | 87.66 | 54.68 |
CK5 | 80 663 | 40.33 M | 71 430 | 29.39 M | 88.55 | 97.39 | 92.63 | 54.17 |
CK6 | 81 281 | 40.64 M | 70 531 | 29.26 M | 86.77 | 97.47 | 92.82 | 54.88 |
HDG1 | 84 427 | 42.21 M | 72 261 | 30.28 M | 85.59 | 97.06 | 91.60 | 54.58 |
HDG2 | 87 332 | 43.67 M | 76 096 | 31.55 M | 87.13 | 97.15 | 91.89 | 54.82 |
HDG3 | 64 752 | 32.38 M | 52 225 | 21.59 M | 80.65 | 87.32 | 73.18 | 55.95 |
HDG4 | 80 293 | 40.15 M | 71 835 | 29.72 M | 89.47 | 96.28 | 89.81 | 54.68 |
HDG5 | 86 806 | 43.40 M | 76 963 | 32.22 M | 88.66 | 97.99 | 93.92 | 55.02 |
HDG6 | 80 087 | 40.04 M | 69 329 | 28.57 M | 86.57 | 98.08 | 94.31 | 54.38 |
组别 Groups | Observed_OTUs | Chao1 | Shannon | Simpson | Goods_coverage |
---|---|---|---|---|---|
CK | 377.000 | 550.657 | 7.287 | 0.985 | 0.908 |
HDG | 350.500 | 544.757 | 6.893 | 0.973 | 0.908 |
Table 4 Alpha diversity index of each group in fecal samples
组别 Groups | Observed_OTUs | Chao1 | Shannon | Simpson | Goods_coverage |
---|---|---|---|---|---|
CK | 377.000 | 550.657 | 7.287 | 0.985 | 0.908 |
HDG | 350.500 | 544.757 | 6.893 | 0.973 | 0.908 |
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