Research Progress in the Biocontrol Secondary Metabolites of Bacillus velezensis

doi:10.13560/j.cnki.biotech.bull.1985.2023-0867

Abstract

Abstract:

Bacillus velezensis is an important representative of biocontrol strains in the genus Bacillus. It is widely used as the core strain of biological pesticide in the biocontrol of plant diseases. B. velezensis has endophytic properties in plants, and its biocontrol mechanism mainly includes three aspects. Firstly, it can produce the secondary metabolites against pathogens. Secondly, it can improve host plant microbial community to promote host nutrition and growth. The last, it can stimulate the plant to produce a defense response and develop systemic resistance. Among them, the production of secondary metabolites is the most important mechanism of biocontrol. B. velezensis contains multiple biosynthesis gene clusters encoding secondary metabolites, including gene clusters encoding polyketide synthase（PKS）and non-ribosomal peptide synthase（NRPS）, as well as gene clusters for ribosomal pathway synthesis of secondary metabolites. B. velezensis can produce plantazolicin, amylocyclicin, and mersacidin through the ribosomal pathway, and produce lipopeptides, polyketides, bacilysin, and bacillibactin through non-ribosomal pathways. These bioactive secondary metabolites have become repositories of natural new drugs and candidate antibiotics, which is of great significance for analyzing the mechanism of biocontrol action. This paper reviews the naming and alteration, the types of secondary metabolites, synthesis and regulation genes, and target pathogens of B. velezensis. It aims to provide a scientific basis for new theories and applications of strain improvement and plant disease biological control.

Key words: Bacillus velezensis, biological control, biological control mechanism, secondary metabolites, secondary metabolite gene cluster

XU Pei-dong, YI Jian-feng, CHEN Di, PAN Lei, XIE Bing-yan, ZHAO Wen-jun. Research Progress in the Biocontrol Secondary Metabolites of Bacillus velezensis[J]. Biotechnology Bulletin, 2024, 40(3): 75-88.

Figures/Tables 4

References 126

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类型 Type	次级代谢产物 Secondary metabolite	防控作用 Controlling effects	靶标病原 Target pathogen	参考文献 Reference
小菌素 Microcin	Plantazolicin	革兰氏阳性细菌Gram-positive bacteria	Bacillus spp.	[34]
		线虫Nematodes		[45]
			Paenibacillus granivorans	[34]
			Micrococcus luteus
			Meloidogyne incognita	[41]
细菌素Bacteriocin	Amylocyclicin	革兰氏阳性细菌Gram-positive bacteria	Bacillus spp.	[35]
			Clavibacter michiganensis
			Micrococcus luteus
			Paenibacillus granivorans
			Paenibacillus polymyxa
			Staphylococcus aureus	[46]
羊毛硫抗生素 Lantibiotic	Mersacidin	细菌Bacteria	Staphylococcus aureus	[48] [50] [56]

类型 Type	次级代谢产物 Secondary metabolite	防控作用 Controlling effects	靶标病原 Target pathogen	参考文献 Reference
小菌素 Microcin	Plantazolicin	革兰氏阳性细菌Gram-positive bacteria	Bacillus spp.	[34]
		线虫Nematodes		[45]
			Paenibacillus granivorans	[34]
			Micrococcus luteus
			Meloidogyne incognita	[41]
细菌素Bacteriocin	Amylocyclicin	革兰氏阳性细菌Gram-positive bacteria	Bacillus spp.	[35]
			Clavibacter michiganensis
			Micrococcus luteus
			Paenibacillus granivorans
			Paenibacillus polymyxa
			Staphylococcus aureus	[46]
羊毛硫抗生素 Lantibiotic	Mersacidin	细菌Bacteria	Staphylococcus aureus	[48] [50] [56]