Progress in the Application of Omics Technology in Biocontrol Bacillus

doi:10.13560/j.cnki.biotech.bull.1985.2024-0498

Abstract

Abstract:

Bacillus is one of the most widely used biocontrol microbial resources in the world. In the era when genomics has become a universal basic discipline, the combined application of transcriptomics, proteomics, metabonomics and other multi-omics technologies have become an important means to deeply reveal the biological characteristics and biocontrol mechanisms of Bacillus. Currently, the NCBI database has published a total of 10 813 genome sequences of Bacillus, of which 1 842 have complete assembly annotations, accounting for 17.04% of the total number. The secondary metabolite gene clusters are the most conserved and specific part of the Bacillus genome. They are not only an important source of natural products, but also play important roles in the interactions and life cycle between bacteria and the environment. Up to now, research on Bacillus has mostly adopted integrated omics models and bioinformatics calculation methods, focusing on the mechanisms of Bacillus resistance formation, biofilm formation, colonization, growth promotion, and induction of plant stress resistance. With the continuous deepening of research, the gene regulatory mechanisms, protein expression, and metabolic pathways in the biocontrol process of Bacillus will be further elucidated, which will help discover new biocontrol active substances and optimize biocontrol strategies. This article reviews the research progress of multi-omics techniques such as genomics, transcriptomics, proteomics, and metabonomics on Bacillus. It aims to provide reference for the analysis of the biocontrol mechanism of Bacillus and the in-depth study of biocontrol strain improvement.

Key words: Bacillus, genomics, transcriptomics, proteomics, metabonomics

XU Pei-dong, YI Jian-feng, CHEN Di, CHEN Hao, XIE Bing-yan, ZHAO Wen-jun. Progress in the Application of Omics Technology in Biocontrol Bacillus[J]. Biotechnology Bulletin, 2024, 40(10): 208-220.

Figures/Tables 1

References 143

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