Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (9): 203-211.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1505

Previous Articles     Next Articles

Effect of Low Crude Protein Diet on the Liver Transcriptome Sequencing of Goats

ZHU Wen(), TANG Ying-ying, SUN Xin-yang, ZHOU Ming, ZHANG Zi-jun, CHEN Xing-yong()   

  1. College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036
  • Received:2020-12-11 Online:2021-09-26 Published:2021-10-25
  • Contact: CHEN Xing-yong E-mail:zhuwen@ahau.edu.cn;chenxingyong@ahau.edu.cn

Abstract:

In order to further apply the low protein diet(LCP)feeding technology and investigate the regulation mechanisms of LCP on goat liver function,RNA-Seq technology was employed to obtain the transcriptome of the liver tissue of Anhui white goat fed LCP or normal diet. Go annotation and KEGG analysis were used to determine the functions of the differentially expressed genes(DEGs),and PPI was employed for the analysis of DEGs interactions. Results showed that there were 62 genes(FDR<0.05)with over two-fold expression variation of genes in the goat liver after LCP feeding,among which 49 genes were up-regulated and 13 genes were down-regulated. A series of DEGs related to protein metabolism,energy and lipid metabolism were identified. The functions of these DEGs were classified into 42 GO items,and the DEGs were mainly concentrated in binding,catalytic activity,molecular transducer activity and other functions. KEGG analysis showed that 23 metabolic pathways were significantly(P<0.05)enriched,including taste transduction,fatty acid biosynthesis,insulin signaling pathway,and so on. Three core DEGs including PPP1R3B,FASN,and IRS2 were enriched in insulin signal pathway in the PPI network. Above results indicate that LCP significantly affects the metabolism of glucose and lipid in the goat liver,increase fatty acid synthesis and lipid peroxidation,and decrease hepatic gluconeogenesis,which provides theoretical basis for the nutrition regulation of low protein diet.

Key words: liver, transcriptomics, low crude protein diet, goat