Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (9): 20-32.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0521
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TAN Bo-wen1,2(), ZHANG Yi2,3, ZHANG Peng2,3, WANG Zhen-yu4(), MA Qiu-xiang2()
Received:
2024-05-30
Online:
2024-09-26
Published:
2024-10-12
Contact:
WANG Zhen-yu, MA Qiu-xiang
E-mail:1050565123@qq.com;wangzy80@126.com;qxma@cemps.ac.cn
TAN Bo-wen, ZHANG Yi, ZHANG Peng, WANG Zhen-yu, MA Qiu-xiang. Identification and Bioinformatics Analysis of Gene in the Magnesium Transporter Family in Cassava[J]. Biotechnology Bulletin, 2024, 40(9): 20-32.
基因Gene | CAAT-box | TATA-box | 光响应元件Light responsive elements | 激素响应元件Hormone responsive elements | 干旱胁迫响应元件Drought stress responsive elements | 低温响应元件Low temperature responsive elements | 其他Others |
---|---|---|---|---|---|---|---|
MeMGT2;1 | 13 | 104 | 23 | 2 | 0 | 1 | 8 |
MeMGT2;2 | 14 | 48 | 15 | 4 | 1 | 3 | 15 |
MeMGT3;1 | 16 | 41 | 6 | 3 | 0 | 0 | 27 |
MeMGT3;2 | 17 | 78 | 14 | 5 | 1 | 0 | 12 |
MeMGT4;1 | 20 | 92 | 9 | 3 | 2 | 0 | 14 |
MeMGT4;2 | 6 | 168 | 14 | 3 | 3 | 1 | 8 |
MeMGT6 | 9 | 111 | 17 | 3 | 0 | 1 | 13 |
MeMGT7 | 7 | 103 | 15 | 6 | 0 | 0 | 13 |
MeMGT9;1 | 10 | 48 | 12 | 5 | 1 | 1 | 20 |
MeMGT9;2 | 17 | 70 | 18 | 5 | 0 | 2 | 9 |
MeMGT9;3 | 22 | 61 | 20 | 4 | 0 | 0 | 17 |
MeMGT10;1 | 16 | 71 | 4 | 9 | 1 | 0 | 27 |
MeMGT10;2 | 13 | 85 | 20 | 6 | 2 | 0 | 20 |
Table 1 Analysis of cis-acting elements of MeMGTs gene family in cassava
基因Gene | CAAT-box | TATA-box | 光响应元件Light responsive elements | 激素响应元件Hormone responsive elements | 干旱胁迫响应元件Drought stress responsive elements | 低温响应元件Low temperature responsive elements | 其他Others |
---|---|---|---|---|---|---|---|
MeMGT2;1 | 13 | 104 | 23 | 2 | 0 | 1 | 8 |
MeMGT2;2 | 14 | 48 | 15 | 4 | 1 | 3 | 15 |
MeMGT3;1 | 16 | 41 | 6 | 3 | 0 | 0 | 27 |
MeMGT3;2 | 17 | 78 | 14 | 5 | 1 | 0 | 12 |
MeMGT4;1 | 20 | 92 | 9 | 3 | 2 | 0 | 14 |
MeMGT4;2 | 6 | 168 | 14 | 3 | 3 | 1 | 8 |
MeMGT6 | 9 | 111 | 17 | 3 | 0 | 1 | 13 |
MeMGT7 | 7 | 103 | 15 | 6 | 0 | 0 | 13 |
MeMGT9;1 | 10 | 48 | 12 | 5 | 1 | 1 | 20 |
MeMGT9;2 | 17 | 70 | 18 | 5 | 0 | 2 | 9 |
MeMGT9;3 | 22 | 61 | 20 | 4 | 0 | 0 | 17 |
MeMGT10;1 | 16 | 71 | 4 | 9 | 1 | 0 | 27 |
MeMGT10;2 | 13 | 85 | 20 | 6 | 2 | 0 | 20 |
基因Gene | 氨基酸数Amino-acid number | 蛋白质分子量Molecular weight/Da | 等电点Isoelectric point | 不稳定系数Instability index | 脂肪指数Aliphaic index | 亚细胞定位Subcellular localization |
---|---|---|---|---|---|---|
MeMGT2;1 | 441 | 5 116.63 | 5.24 | 61.16 | 99.71 | Plasma membrane |
MeMGT2;2 | 443 | 50 084.46 | 5.17 | 60.79 | 99.68 | Plasma membrane |
MeMGT3;1 | 419 | 46 929.10 | 5.31 | 41.71 | 105.89 | Plasma membrane |
MeMGT3;2 | 420 | 47 276.18 | 5.15 | 39.36 | 100.98 | Plasma membrane |
MeMGT4;1 | 495 | 55 072.03 | 4.85 | 41.36 | 91.23 | Cytoplasmic |
MeMGT4;2 | 496 | 55 057.13 | 4.94 | 42.90 | 89.46 | Cytoplasmic |
MeMGT6 | 449 | 50 165.42 | 5.59 | 48.10 | 100.56 | Chloroplast |
MeMGT7 | 391 | 44 564.62 | 4.87 | 34.30 | 102.23 | Golgi apparatus |
MeMGT9;1 | 400 | 44 715.05 | 5.07 | 40.07 | 99.27 | Plasma membrane |
MeMGT9;2 | 402 | 44 505.71 | 4.92 | 41.99 | 100.00 | Chloroplast |
MeMGT9;3 | 423 | 47 245.86 | 5.06 | 51.35 | 97.09 | Cytoplasmic |
MeMGT10;1 | 444 | 49 771.50 | 5.82 | 54.37 | 106.01 | Plasma membrane |
MeMGT10;2 | 447 | 50 089.05 | 5.75 | 54.74 | 107.25 | Plasma membrane |
Table 2 Physicochemical properties of MeMGTs proteins in cassava
基因Gene | 氨基酸数Amino-acid number | 蛋白质分子量Molecular weight/Da | 等电点Isoelectric point | 不稳定系数Instability index | 脂肪指数Aliphaic index | 亚细胞定位Subcellular localization |
---|---|---|---|---|---|---|
MeMGT2;1 | 441 | 5 116.63 | 5.24 | 61.16 | 99.71 | Plasma membrane |
MeMGT2;2 | 443 | 50 084.46 | 5.17 | 60.79 | 99.68 | Plasma membrane |
MeMGT3;1 | 419 | 46 929.10 | 5.31 | 41.71 | 105.89 | Plasma membrane |
MeMGT3;2 | 420 | 47 276.18 | 5.15 | 39.36 | 100.98 | Plasma membrane |
MeMGT4;1 | 495 | 55 072.03 | 4.85 | 41.36 | 91.23 | Cytoplasmic |
MeMGT4;2 | 496 | 55 057.13 | 4.94 | 42.90 | 89.46 | Cytoplasmic |
MeMGT6 | 449 | 50 165.42 | 5.59 | 48.10 | 100.56 | Chloroplast |
MeMGT7 | 391 | 44 564.62 | 4.87 | 34.30 | 102.23 | Golgi apparatus |
MeMGT9;1 | 400 | 44 715.05 | 5.07 | 40.07 | 99.27 | Plasma membrane |
MeMGT9;2 | 402 | 44 505.71 | 4.92 | 41.99 | 100.00 | Chloroplast |
MeMGT9;3 | 423 | 47 245.86 | 5.06 | 51.35 | 97.09 | Cytoplasmic |
MeMGT10;1 | 444 | 49 771.50 | 5.82 | 54.37 | 106.01 | Plasma membrane |
MeMGT10;2 | 447 | 50 089.05 | 5.75 | 54.74 | 107.25 | Plasma membrane |
Fig. 4 Evolutionary tree of MGT gene family members of Manihot esculenta crantz, Hevea brasiliensis, Arabi-dopsis thaliana, Nicotiana tabacum, Glycine max, and Solanum lycopersicum
Fig. 7 Conservative analysis of MeMGTs proteins in cassava The black box in the figure is marked with the tripeptide motif Gly-Met-Asn in the conserved region of the magnesium transporter
基因Gene | 基因ID Gene ID | MeMGTs基因家族蛋白质二级结构预测Prediction of secondary structure of MeMGTs gene family proteins/% | ||||
---|---|---|---|---|---|---|
α-螺旋α-helix | 延伸链Extended strand | β-转角β-turn | 无规则卷曲Random coil | |||
MeMGT2;1 | Manes.11G068600 | 53.74 | 6.80 | 2.04 | 37.41 | |
MeMGT2;2 | Manes.04G101500 | 51.02 | 7.45 | 2.93 | 38.60 | |
MeMGT3;1 | Manes.05G110400 | 54.89 | 7.16 | 2.63 | 35.32 | |
MeMGT3;2 | Manes.01G027400 | 54.05 | 7.62 | 2.38 | 35.95 | |
MeMGT4;1 | Manes.07G053800 | 52.93 | 5.45 | 2.22 | 39.39 | |
MeMGT4;2 | Manes.10G084800 | 55.44 | 4.64 | 1.21 | 38.71 | |
MeMGT6 | Manes.07G020300 | 50.78 | 6.68 | 2.23 | 40.31 | |
MeMGT7 | Manes.05G085100 | 57.80 | 8.44 | 3.32 | 30.43 | |
MeMGT9;1 | Manes.03G169600 | 60.00 | 7.50 | 2.50 | 30.00 | |
MeMGT9;2 | Manes.15G036500 | 59.20 | 6.97 | 3.23 | 30.60 | |
MeMGT9;3 | Manes.15G036400 | 56.50 | 9.22 | 4.26 | 30.02 | |
MeMGT10;1 | Manes.11G155200 | 47.52 | 8.33 | 2.93 | 41.22 | |
MeMGT10;2 | Manes.04G011000 | 51.01 | 7.38 | 2.46 | 39.15 |
Table 3 Composition of predicted secondary structure among MeMGTs proteins in cassava
基因Gene | 基因ID Gene ID | MeMGTs基因家族蛋白质二级结构预测Prediction of secondary structure of MeMGTs gene family proteins/% | ||||
---|---|---|---|---|---|---|
α-螺旋α-helix | 延伸链Extended strand | β-转角β-turn | 无规则卷曲Random coil | |||
MeMGT2;1 | Manes.11G068600 | 53.74 | 6.80 | 2.04 | 37.41 | |
MeMGT2;2 | Manes.04G101500 | 51.02 | 7.45 | 2.93 | 38.60 | |
MeMGT3;1 | Manes.05G110400 | 54.89 | 7.16 | 2.63 | 35.32 | |
MeMGT3;2 | Manes.01G027400 | 54.05 | 7.62 | 2.38 | 35.95 | |
MeMGT4;1 | Manes.07G053800 | 52.93 | 5.45 | 2.22 | 39.39 | |
MeMGT4;2 | Manes.10G084800 | 55.44 | 4.64 | 1.21 | 38.71 | |
MeMGT6 | Manes.07G020300 | 50.78 | 6.68 | 2.23 | 40.31 | |
MeMGT7 | Manes.05G085100 | 57.80 | 8.44 | 3.32 | 30.43 | |
MeMGT9;1 | Manes.03G169600 | 60.00 | 7.50 | 2.50 | 30.00 | |
MeMGT9;2 | Manes.15G036500 | 59.20 | 6.97 | 3.23 | 30.60 | |
MeMGT9;3 | Manes.15G036400 | 56.50 | 9.22 | 4.26 | 30.02 | |
MeMGT10;1 | Manes.11G155200 | 47.52 | 8.33 | 2.93 | 41.22 | |
MeMGT10;2 | Manes.04G011000 | 51.01 | 7.38 | 2.46 | 39.15 |
基因Gene | 基因ID Gene ID | MeMGTs基因家族蛋白质磷酸化位点数量Number of phosphorylation sites of MeMGTs gene family proteins | ||
---|---|---|---|---|
丝氨酸Ser | 苏氨酸Thr | 酪氨酸Tyr | ||
MeMGT2;1 | Manes.11G068600 | 33 | 9 | 4 |
MeMGT2;2 | Manes.04G101500 | 33 | 11 | 3 |
MeMGT3;1 | Manes.05G110400 | 18 | 15 | 4 |
MeMGT3;2 | Manes.01G027400 | 22 | 14 | 4 |
MeMGT4;1 | Manes.07G053800 | 16 | 20 | 3 |
MeMGT4;2 | Manes.10G084800 | 25 | 25 | 3 |
MeMGT6 | Manes.07G020300 | 22 | 13 | 3 |
MeMGT7 | Manes.05G085100 | 22 | 17 | 6 |
MeMGT9;1 | Manes.03G169600 | 19 | 13 | 3 |
MeMGT9;2 | Manes.15G036500 | 19 | 12 | 5 |
MeMGT9;3 | Manes.15G036400 | 22 | 12 | 2 |
MeMGT10;1 | Manes.11G155200 | 27 | 10 | 3 |
MeMGT10;2 | Manes.04G011000 | 31 | 7 | 4 |
Table 4 Number of phosphorylation sites of MeMGTs proteins in cassava
基因Gene | 基因ID Gene ID | MeMGTs基因家族蛋白质磷酸化位点数量Number of phosphorylation sites of MeMGTs gene family proteins | ||
---|---|---|---|---|
丝氨酸Ser | 苏氨酸Thr | 酪氨酸Tyr | ||
MeMGT2;1 | Manes.11G068600 | 33 | 9 | 4 |
MeMGT2;2 | Manes.04G101500 | 33 | 11 | 3 |
MeMGT3;1 | Manes.05G110400 | 18 | 15 | 4 |
MeMGT3;2 | Manes.01G027400 | 22 | 14 | 4 |
MeMGT4;1 | Manes.07G053800 | 16 | 20 | 3 |
MeMGT4;2 | Manes.10G084800 | 25 | 25 | 3 |
MeMGT6 | Manes.07G020300 | 22 | 13 | 3 |
MeMGT7 | Manes.05G085100 | 22 | 17 | 6 |
MeMGT9;1 | Manes.03G169600 | 19 | 13 | 3 |
MeMGT9;2 | Manes.15G036500 | 19 | 12 | 5 |
MeMGT9;3 | Manes.15G036400 | 22 | 12 | 2 |
MeMGT10;1 | Manes.11G155200 | 27 | 10 | 3 |
MeMGT10;2 | Manes.04G011000 | 31 | 7 | 4 |
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