Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (9): 20-32.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0521

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Identification and Bioinformatics Analysis of Gene in the Magnesium Transporter Family in Cassava

TAN Bo-wen1,2(), ZHANG Yi2,3, ZHANG Peng2,3, WANG Zhen-yu4(), MA Qiu-xiang2()   

  1. 1. School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228
    2. National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Science, Shanghai 200032
    3. University of Chinese Academy of Sciences, Beijing 100049
    4. Institute of Nanfan & Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316
  • Received:2024-05-30 Online:2024-09-26 Published:2024-10-12
  • Contact: WANG Zhen-yu, MA Qiu-xiang E-mail:1050565123@qq.com;wangzy80@126.com;qxma@cemps.ac.cn

Abstract:

【Objective】Magnesium transporters(MGTs)are vital for magnesium transportation,thus analyzing the basic information, the physiological and chemical characteristics of the MeMGT gene family in cassava serves as a foundational step for understanding the functions of these genes.【Method】In this study, we identified and named 13 putative MeMGTs genes in cassava(Manihot esculenta Crantz)based on the cassava genome database through homologous sequence alignments, designated as MeMGT2;1-MeMGT10;2. We conducted chromosomal localization, conserved motif analysis, and phylogenetic analysis of the MeMGT family genes. Additionally, we predicted the biochemical properties, structure, and function of the corresponding proteins. 【Result】Chromosomal analysis revealed that the 13 MeMGTs genes were distributed across 8 chromosomes, each containing 4 to 13 exons. Tissue-specific expression patterns indicated differential expressions of MeMGTs genes across various developmental stages. MeMGT4;2 was mainly expressed in cassava fibrous root and storage root, which might potentially play a role in storage root development, and MeMGT7 was mainly expressed in cassava leave, vein and stem with function possibly involved in long-distance Mg2+ transport. Cis-acting element analysis revealed the presence of numerous photoresponsive and hormone-responsive elements in the promoter regions of MeMGTs genes, suggesting regulation by multiple factors. Phylogenetic analysis of the MGT gene family categorized these genes into five distinct clusters between cassava and selected model species. Protein sequence analysis identified MeMGTs member proteins as hydrophilic proteins with two conserved transmembrane domains and a Gly-Met-Asn(GMN)tripeptide motif, devoid of signal peptides. Phosphorylation analysis indicated the presence of phosphorylation sites, including serine, threonine, and tyrosine sites, which may contribute to the functionality of MeMGTs member proteins.【Conclusion】MeMGTs genes are regulated by various elements including light, hormone, drought and cold responses, might play a crucial role in the growth, development and adversity stress of cassava.

Key words: cassava, magnesium transporter, MeMGT gene family, gene identification, bioinformatics