Genome-wide Identification and Expression Pattern Analysis of BZR Transcription Factor Gene Family of Melon

doi:10.13560/j.cnki.biotech.bull.1985.2024-0162

Abstract

Abstract:

【Objective】 Brassinazole-resistant（BZR）is a plant specific transcription factor that plays a crucial role in plant growth and development. Identifying members of the CmBZR gene family across the whole melon genome, and analyzing the expression patterns of related gene, may provide a basis for further exploring the biological functions of the melon BZR gene family. 【Method】 Based on the whole melon genome data, the BZR family genes in melon were identified using BLAST. Bioinformatics methods were used to analyze the physicochemical properties, chromosomal distribution, gene structure, protein domains, phylogenetic evolution, and cis acting elements of the family proteins. Fluorescence quantitative PCR was used to verify the expression of BZR family genes in different tissues and fruits of melon at different growth and development stages. 【Result】 A total of 6 BZR genes were identified in cantaloupe, which can be divided into 5 subfamilies through phylogenetic analysis. CmBZR genes are distributed on chromosomes 3, 7, 8, 11, and 12. All BZR proteins have conserved domains. The promoter region of the CmBZR gene is significantly enriched with cis-acting elements related to growth and development, hormone signal transduction, and abiotic stress. CmBEH1-4 is highly expressed in stems, female flowers, and ovaries, and is also expressed in growth, maturity, respiratory climacteric, and late respiratory climacteric stages. 【Conclusion】 Six members of the melon CmBZR gene family have been systematically identified across the whole genome, and different genes are expressed in various tissues and growth stages of the melon，and their expression patterns are various.

Key words: melon（Cucumis melo L.）, BZR, transcription factor, genome-wide identification, expression analysis, bioinformatics

ZANG Wen-rui, MA Ming, CHE Gen, HASI Agula. Genome-wide Identification and Expression Pattern Analysis of BZR Transcription Factor Gene Family of Melon[J]. Biotechnology Bulletin, 2024, 40(7): 163-171.

Figures/Tables 6

Table 1 Basic information of BZR family members in melon

基因名称 Gene name	登录号Accession No.	染色体分布Chromosome distribution	染色体座位Chromosomal loci/bp	ORF /bp	氨基酸数量Number of amino acids	分子量Molecular weight/kD	等电点Isoelectric point（pI）
CmBZR1	MELO3C010925.2.1	Chr 03	29999092-30000475	936	311	34.05	9.16
CmBES1	MELO3C007804.2.1	Chr 08	5468519-5471484	960	319	34.71	8.96
CmBEH1	MELO3C016213.2.1	Chr 07	22268773-22275513	1 932	643	75.11	5.90
CmBEH2	MELO3C021214.2.1	Chr 11	31280641-31285843	2 046	681	78.21	5.74
CmBEH3	MELO3C016121.2.1	Chr 07	20822745-20826732	978	325	34.70	8.50
CmBEH4	MELO3C002681.2.1	Chr 12	22058946-22061841	984	327	39.52	9.00

Fig. 1 Analysis of CmBZR protein sequences A: Multiple sequence alignment of 6 CmBZR proteins; B: conserved sites of BZR domains in CmBZR sequences. *: Conserved motif of CmBZR protein sequences

Fig. 2 Gene structure analysis of BZR genes in melon

Fig. 3 Phylogenetic tree analysis of BZR proteins from Arabidopsis，Oryza sativa，Solanum lycopersicon，Cucumis sativus，Cucurbita moschata and C. smelo I-V indicates different subfamily of BZR members in six species

Fig. 4 Cis-elements of promoter region of CmBZR gene family in melon

Fig. 5 Expression analysis of CmBZR in melon A: Heat map of 6 CmBZR genes expressed differently in different tissues of melon；B: expression profiles of CmBZR genes in melon’s Rt, S, L, FF, MF, O, G,R, C and P, and they indicate the root, stem, leaf, female flower, male flower, ovary, growing stage, ripening stage, climacteric stage, and post-climacteric stage fruit, respectively.

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