Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (8): 142-151.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0257
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ZHOU Lin1(), HUANG Shun-man1, SU Wen-kun1, YAO Xiang2, QU Yan1()
Received:
2024-03-16
Online:
2024-08-26
Published:
2024-07-31
Contact:
QU Yan
E-mail:1617574776@qq.com;quyan@swfu.edu.cn
ZHOU Lin, HUANG Shun-man, SU Wen-kun, YAO Xiang, QU Yan. Identification of the bHLH Gene Family and Selection of Genes Related to Color Formation in Camellia reticulata[J]. Biotechnology Bulletin, 2024, 40(8): 142-151.
基因Gene | 上游引物Forward primer(5'-3') | 下游引物Reverse primer(5'-3') |
---|---|---|
CrbHLH8 | GCAGTTGTGCCCTCAACAAG | CACTCACCTCCATTGGCTGT |
CrbHLH13 | CGGCTGAATGCTCGTTGATG | CTGAAATTGGCGGTGTTGGG |
CrbHLH59 | ACCCCATTGAATTCCCCACC | TACAAATCCAGGATCCGCGG |
CrbHLH61 | GCAGTTGTGCCCTCAACAAG | CACTCACCTCCATTGGCTGT |
CrbHLH63 | TGTCCATTGGATGCTCACCC | CAGCACCATCTTGAGTCCGT |
CrbHLH71 | AGCCACGTACTGCTGAACAA | TCTGCGCTATTGCCAGAACA |
CrActin | GTCTGTTCCCTCTCCTCCCT | GAAGGGATCGTTGACAGCGA |
Table 1 Primers used for RT-qPCR
基因Gene | 上游引物Forward primer(5'-3') | 下游引物Reverse primer(5'-3') |
---|---|---|
CrbHLH8 | GCAGTTGTGCCCTCAACAAG | CACTCACCTCCATTGGCTGT |
CrbHLH13 | CGGCTGAATGCTCGTTGATG | CTGAAATTGGCGGTGTTGGG |
CrbHLH59 | ACCCCATTGAATTCCCCACC | TACAAATCCAGGATCCGCGG |
CrbHLH61 | GCAGTTGTGCCCTCAACAAG | CACTCACCTCCATTGGCTGT |
CrbHLH63 | TGTCCATTGGATGCTCACCC | CAGCACCATCTTGAGTCCGT |
CrbHLH71 | AGCCACGTACTGCTGAACAA | TCTGCGCTATTGCCAGAACA |
CrActin | GTCTGTTCCCTCTCCTCCCT | GAAGGGATCGTTGACAGCGA |
Fig. 3 Analysis of conserved motif and conserved domain of the bHLH gene family of C. reticulate A: Cluster analysis of bHLH gene family members of C. reticulata. B: Conserved motif analysis of bHLH gene members of C. reticulata. C: Conserved domain of the bHLH gene family members of C. reticulata
Fig. 4 Differential bHLH analysis of C. reticulate A: Veen map of C. reticulata varieties. B: Veen map of C. reticulata at different developmental stages. C: Differential bHLH gene combination heat map. S: C. reticulata ‘Shizitou’. T: C. reticulata ‘Tongzimian’. 1: Bud stage. 2: Full flowering stage. -1, -2, and -3 indicate three repetitions. The same below
Fig. 5 Floral phenotype(A), and key pigment content(B)in C. reticulata ‘Shizitou’ and C. reticulata ‘Tongzimian’ Different lower letters indicate significant differences at P<0.05 level. The same below
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