Fig. 1 Inositol biosynthesis pathway using fructose and glucose as substrateglf: Glucose facilitator gene. glk: Glucokinase gene. imp: Inositol monophosphatase gene. Scips: Inositol-3-phosphate synthase gene derived from Saccharomyces cerevisiae. pgi: Glucose-6-phosphate isomerase gene. zwf: Glucose-6-phosphate dehydrogenase gene. tktA: Transketolase gene. fucP: Fructose transporter gene. mak: Fructokinase gene. Scshb17: Sedoheptulose bisphosphatase gene. Cgeno: Enolase gene derived from Corynebacterium glutamicum

Fig. 2 Phenotype verification of recombinant strain JY1 and JY2A: Growth curves of JY1 strain cultured in the medium containing different carbon sources. 10G: Fermentation medium contains 10.0 g/L glucose. 10F: Fermentation medium contains 10.0 g/L fructose. 5G5F: Fermentation medium contains 5.0 g/L glucose and 5.0 g/L fructose. B: Growth curve and inositol production of JY2 strain cultured in the medium containing mixed carbon source

Fig. 3 Phenotype verification of recombinant strain JY3-JY10A: Growth curves of recombinant strains cultured in the medium containing mixed carbon source. B: Inositol production, carbon source concentration and maximum OD600 of recombinant strains cultured in the medium containing mixed carbon source

Fig. 4 Fermentation results of recombinant strains JY12-JY14 in 3 L reactorA: Fermentation results of JY12 strain in 3 L reactor. B: Fermentation results of JY13 strain in 3 L reactor. C: Fermentation results of JY14 strain in 3 L reactor

Fig. 5 Establishment and stability evaluation of Scips, imp and eno synergetic expression systemA: P9 plasmid map and CAST system. B: Stability verification of gene editing and P9 expression plasmids

Fig. 6 Shake flask fermentation results of recombinant strain JY17A: Growth curve and inositol production during fermentation without adding antibiotics. B: Glucose and fructose consumption during fermentation without adding antibiotic. C: Growth curve and inositol production during fermentation with adding antibiotic. D: Glucose and fructose consumption during fermentation with adding antibiotic

Fig. 7 Fermentated results of recombinant strain JY17 and JY18 in 3 L reactorA: Growth curve and inositol production of JY17. B: Glucose and fructose consumption of JY17. C: Growth curve and inositol production of JY18. D: Glucose and fructose consumption of JY18