Technology of RNAi and Its Application in Plant Research

Abstract

Abstract: RNA interference（RNAi）belongs to the post-transcriptional gene silencing phenomenon in which double-stranded RNA triggers the degradation of homologous mRNA, thereby specific to suppression the corresponding gene expression. RNAi is a phenomenon that commonly existed in organisms, and it has become one of the hotspots of scientific research in 21st century. In recent years, RNAi plays a more and more important role in plant functional genomics. We reviewed characteristics and action mechanism of RNAi, as well as the research progress in aspects including the plant gene function analysis, the crop variety improvement and the resistance to disease or pests, in order to provide reference for RNAi application in plant research.

Key words: RNA interference, Double-stranded RNA, Gene silencing, Plant, Application

Wang Tingting, Wang Dandan, Rahman Laibi Chelab, Kang Dan, You Tengfei, Sui Anping, Yang Xingyong. Technology of RNAi and Its Application in Plant Research[J]. Biotechnology Bulletin, 2013, 0(3): 48-52.

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