Abstract: It was to clone mcl-1（myeloid cell leukemia-1）gene from Bufo japonicus formosus and analyze the sequence of the gene. The plasmid cDNA library of adult Japanese toad Bufo japonicus formosus skin was screened by bacterial colony PCR. The sequence was analyzed by bioinformatics method, and the structure and function of MCL-1 were predicted. Result showed that the full length cDNA of mcl-1（GenBank accession number ：JX219482）was 1 090 bp. The 5'- and 3'-untranslated regions are 19 bp and 432 bp, respectively, while the ORF is 639 bp encoding 212 amino acid residues. Homologous analysis indicated the similarity of Bufo japonicus formosus with Xenopus laevis was 60%, and 38%-55% with other species. The phylogenetic tree of MCL-1 amino acid sequences was consistent with morphological taxonomic characters. The analysis indicates that the structural characteristics of MCL-1 of B. japonicus formosus seem to be similar with those of human. This report would become the basis for the further studies on MCL-1.

Key words: Bufo japonicus formosus, MCL-1 Gene cloning, Sequence analysis