Biological Effects of Simulated Microgravity on Arabidopsis Seedlings

Abstract

Abstract: Under normal conditions and simulated microgravity on the growth of Arabidopsis seedlings, the experimental results show that simulated microgravity can develop the following results：(1) affects the growth and development of Arabidopsis seedlings；(2) simulated microgravity influences Arabidopsis photosynthetic characteristics；(3) simulated microgravity affect physiological and biochemical indices of Arabidopsis；(4) simulated microgravity affects the calcium distribution of Arabidopsis. In addition, we use gene chip technology to investigate the influence of simulated microgravity on the Arabidopsis gene expression.

Key words: Simulated microgravity, Gene chip, Gene expression

Wu Chengfei, Guo Shuangsheng, Zhao Qi, Sun Jianfeng. Biological Effects of Simulated Microgravity on Arabidopsis Seedlings[J]. Biotechnology Bulletin, 2013, 0(5): 58-62.

References

[1] 王浩, 牛颜冰.航天育种机理的研究进展[J]. 山西农业大学学报：自然科学版, 2009, 29(2)：120-121.
[2] Albrecht-Buehler G. The simulation of microgravity conditions on the ground[J]. ASGSB Bulletin, 1992, 5(2)：3-10.
[3] Sobol M, Kordyum E. Distribution of calcium ions in cells of the root distal elongation zone under clinorotation[J]. Microgravity Sci Technol, 2009, 21(1-2)：179-185.
[4] Zhao Q, Li J, Liu M. Effects of simulated microgravity on characteris-tics of photosynthesis in plant seedling[J]. Space Medicine & Me-dical Engineering, 2002, 15(2)：79-83.
[5] 郑景生.微重力下的植物生物学效应与空间诱变育种研究[J]. 江西农业大学学报：自然科学版, 2003, 25(5) ：671-675.
[6] 费翅鲲, 胡向阳, 崔大勇, 等.模拟微重力的植物生物学效应[J]. 自然科学进展, 2002, 12(2) ：135-139.
[7] 刘敏, 王亚林, 薛淮, 等.模拟微重力条件下植物细胞亚显微结构的研究[J]. 航天医学与医学工程, 1999, 12(5)：360-363.
[8] 黄伟, 黄晓兵, 郭梅, 等.模拟微重力对玉米根尖细胞核的影响[J].内江师范学院学报, 2008, 23(4)：57-59.
[9] Hilaire E, Guikema JA, Brown J, Gravit CS. Clinorotation affects soybean seedling morphology[J]. Physiology, 1995, 2：149-150.
[10] Kochba J, Lvee S. Differences is peroxidase activity and isoenzymes in embryogenic and non-embryogenic shamouti orange ovular callus lines[J]. Plant Cell Physiology, 1977, 18：463-467.
[11] Cai WM. Isozyme analysis of SOD of several plants species in simu-late microgravity condition[J]. Acta Phytophysiologica Sinica, 2000, 26：137-142.
[12] Hepler PK, Wayne RO. Calcium and plant development[J]. Annu Rev Plant Physiol, 1985, 36：397-439.
[13] Zhao HC, Zhu T, Wu J, Xi BS. Effect of simulated microgravity on aged pea seed vigour and related physiological properties[J]. Colloids and Surfaces B：Biointerfaces, 2003, 27：311-314.
[14] Gilroy S, Jones RL. Gibberellic acid and abscisic acid coordinately regulate cytoplasmic calcium and secretory activity in barley aleu-rone protoplasts[J]. Proc Natl Acad Sci USA, 1992, 89(8)：3591-3995.
[15] Hilaire E, Paulsen AO, Brown CS, et al. Microgravity and clinorotation cause redistribution of free calcium in sweet clover columella cells[J]. Plant Cell Physiol, 1995, 36：831-837.
[16] 徐继, 赵琦. 微重力对石刁柏根尖组织和细胞中钙水平及分布的影响[J]. 生物物理学报, 1999, 15(2)：381-386.
[17] Salmi ML, Roux SJ. Gene expression changes induced by space flight in single-cells of the fern Ceratopteris richardii[J]. Planta, 2008, 229：151-159.
[18] Centis-Aubay S, Gasset G, Mazars C, et al. Changes in gravitational forces induce modifications of gene expression in A. thaliana seedlings[J]. Planta, 2003, 218：179-185.
[19] Commission on Physical Sciences Mathematics and Applications National Research Council. Biological sciences and biotechnology microgravity research opportunities for the 1990s[R]. Conmittee on Microgravity Research Space Studies Board, 1999：62-75.
[20] Barjaktarovi? ?, Babbick M, Nordheim A, et al. Alterations in protein expression of Arabidopsis thaliana cell cultures during hyper- and simulated micro-gravity[J]. Microgravity Sci Technol, 2009, 21：191-196.