Molecular Cloning and Expression Analysis of ERF Transcription Factor Gene in Gardenia jasminoides During Fruit Development

Abstract

Abstract: Ethylene-responsive element-binding factor（ERF）genes constitute one of the DNA-binding protein gene families in plants, and are transcription factors associated with ethylene signal transduction, developmental and physiological processes. In this paper, a new member of the AP2/ERF transcription factor family, GjERF, was isolated from Gardenia jasminoides fruit cDNA library. The GjERF cDNA is 962 bp, contains a predicated 780 bp open reading frame that encodes a protein of 259 amino acids with calculated molecular mass of 28.6 kD, and with a 82 bp non-coding region at 5' end, a 100 bp of non-coding region flank at 3' end. Sequence analysis showed that GjERF contained an AP2/ERF domain of 59 amino acids and a N- terminal MC motif（MCGGAII）. It belonged to a group Ⅶ protein in the ERF subfamily. The RT-PCR analysis indicated that the transcription of GjERF gene accumulated primarily in mature leaves of G.jasminoides, and was not related with fruit developmental stages.

Key words: Gardenia jasminoides, ERF, Clone, Expression

Gao Lan, Lu Jingwen. Molecular Cloning and Expression Analysis of ERF Transcription Factor Gene in Gardenia jasminoides During Fruit Development [J]. Biotechnology Bulletin, 2013, 0(7): 60-65.

References

[1] Liu J, Li J, et al. Identification and expression analysis of ERF trans-cription factor genes in petunia during flower senescence and in resp-onse to hormone treatments[J]. J Exp Bot, 2011, 62：825-840.
[2] Li Y, Zhu B, et al. LeERF1 positively modulated ethylene triple resp-onse on etiolated seedling, plant development and fruit ripening and softening in tomato[J]. Plant Cell Rep, 2007, 26（11）：1999-2008.
[3] Zhou J, Tang X, Martin GB. The Pto kinase conferring resistance to tomato bacterial speck disease interacts with proteins that bind a cis-element of pathogenesis-related genes[J]. EMBO J, 1997, 16（11）：3207-3218.
[4] Akagi A, Dandekar AM, Stotz HU. Resistance of Malus domestica Fruit to Botrytis cinerea depends on endogenous ethylene biosynthesis[J]. Phytopathology, 2011, 101（11）：1311-1321.
[5] Gibbs DJ, Lee SC, Isa NM, et al. Homeostatic response to hypoxia is regulated by the N-end rule pathway in plants[J]. Nature, 2011, 479（7373）：415-418.
[6] Shinozaki K, Yamaguchi-Shinozaki K, Seki M. Regulatory network of gene expression in the drought and cold stress responses[J]. Curr Opin Plant Biol, 2003, 6（5）：410-417.
[7] Ohme-Takagi M, Shinshi, H. Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element[J]. Plant Cell, 1995, 7（2）：173-182.
[8] Hao D, Ohme-Takagi M, Sarai A. Unique mode of GCC box recogni-tion by the DNA-binding domain of ethylene-responsive element-binding factor（ERF domain）in plant[J]. J Biol Chem, 1998, 273（41）：26857-6861.
[9] Fischer U, Dr?ge-Laser W. Over expression of NtERF5, a new member of the tobacco ethylene response transcription factor family enhances resistance to tobacco mosaic virus[J]. Mol Plant Microbe Interact, 2004, 17（10）：1162-1171.
[10] O?ate-Sánchez L, Singh KB. Identification of Arabidopsis ethylene-responsive element binding factors with distinct induction kinetics after pathogen infection[J]. Plant Physiol, 2002, 128（4）：1313-1322.
[11] Tournier B, Sanchez-Ballesta MT, Jones B, et al. New members of the tomato ERF family show specific expression pattern and diverse DNA-binding capacity to the GCC box element[J]. FEBS Lett, 2003, 550（1-3）：149-154.
[12] Nakano T, Suzuki K, Fujimura T, et al. Genome-wide analysis of the ERF gene family in Arabidopsis and rice[J]. Plant Physiol, 2006, 140（2）：411-432.
[13] Zhang G, Chen M, Chen X, et al. Phylogeny, gene structures, and expression patterns of the ERF gene family in soybean（Glycine max L.）[J]. J Exp Bot, 2008, 59（15）：4095-4107.
[14] Okamuro JK, Caster B, et al. The AP2 domain of APETALA2 def-ines a large new family of DNA binding proteins in Arabidopsis[J]. Proc Natl Acad Sci USA, 1997, 94（13）：7076-7081.
[15] Allen MD, Yamasaki K, Ohme-Takagi M, et al. A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA[J]. EMBO J, 1998, 17（18）：5484-5496.
[16] Sakuma Y, Liu Q, Dubouzet JG, et al. DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression[J]. Biochem Biophys Res Commun, 2002, 290（3）：998-1009.
[17] Bekesiova I, Nap JP, Mlynarova L. Isolation of high quality DNA and RNA from Leaves of the carnivorous plant Drosera rotundifolia[J]. Plant Mol Biol Rep, 1999, 17（3）：269-277.
[18] Allen MD, Yamasaki K, Ohme-Takagi M, et al. A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA[J]. EMBO J, 1998, 17（18）：5484-5496,
[19] Fujimoto SY, Ohta M, Usui A, et al. Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression[J]. Plant Cell, 2000, 12（3）：393-404.
[20] Zhang Z, Zhang H, Quan R, et al. Transcriptional regulation of ethylene response factor LeERF2 in the expression of ethylene biosynthesis genes controls ethylene production in tomato and tobacco[J]. Plant Physiol, 2009, 150（1）：365-377.
[21] Wang A, Tan D, Takahashi A, et al. MdERFs, two ethylene-response factors involved in apple fruit ripening[J]. J Exp Bot, 2007, 58（13）：3743-3748.
[22] El-Sharkawy I, Sherif S, Mila I, et al. Molecular characterization of seven genes encoding ethylene-responsive transcriptional factors during plum fruit development and ripening[J]. J Exp Bot, 2009, 60（3）：907-922.
[23] Yin XR, Allan AC, Chen KS, et al. Kiwifruit EIL and ERF genes involved in regulating fruit ripening[J]. Plant Physiol, 2010, 153（3）：1280-1292.
[24] Licausi F, Giorgi FM, Zenoni S, et al. Genomic and transcriptomic analysis of the AP2/ERF superfamily in Vitis vinifera[J]. BMC Genomics, 2010, 11：719.
[25] 高峰, 怡荣, 郝金凤, 等.甜瓜乙烯应答因子基因在果实发育成熟过程中的表达特性[J]. 西北植物学报, 2012, 32（5）：886-889.