Yak HSFY Gene Cloning and Structure Anlysis

Abstract

Abstract: Heat shock transcription factor linked Y chromosome is one of the candidate genes for spermatogenesis obstacle. In this study, sequences of HSFY genes of yak were obtained by cloning and alignmented with 84 HSFY sequences on the Y chromosome of the cow. The results show that：The nucleotide homology of yak, HSFY genes which are composed with two exons and one intron with the corresponding sequences in the genome of the cow is high up to 99.54%. We found four insertion/deletion sequences. Between the 1 012-1 020 bp（Ⅰ）, it lacks 9 nucleotides（AAAGAAGA/TG）which is located in the intron. While between the 1 071-1 073 bp（Ⅱ）and 1 249-1 251 bp（Ⅲ）lack AAG and CCA/C respectively, which are located in the second extron and that lack lysine and histidine respectively in the amino acid sequence. Between the 1 708-1 710 bp（Ⅳ）, there is a 3 nucleotides deletion（CAA）, which is located in 3'-URT. In the 84 HSFY genes of the cow, the frequency of the insertions/deletion is 29，3，2，1 forⅠ，Ⅱ，Ⅲ and Ⅳ, respectively. It is not found one with both Ⅱ and Ⅲ deletion. But all Ⅱ or Ⅲ deletion are accompany withⅠdeletion.Ⅱ and Ⅲ deletions change three-dimensional structure of HSFY protein. Yak HSFY which is a multicopy gene prefers using codes that are ended with A or T.

Key words: Yak, HSFY Insertion/deletion, Spermatogenesis

Zeng Xianbin,Wang Yong,Liu Zhongna,Ma Zhijie,Hai Ting,Zhong Jincheng. Yak HSFY Gene Cloning and Structure Anlysis[J]. Biotechnology Bulletin, 2013, 0(7): 82-88.

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