Clone and Function Analysis MiR160f in Common Wild Rice(Oryza rufipogon Griff.)

Abstract

Abstract: MicroRNAs are a class of non-coding RNAs involved in post- transcriptional control of gene expression. Former study on Arabidopsis thaliana reveals that miR160 involves in root cell division and differentiation by regulating auxin response factors（ARF）thus influence the root development. This study cloned miR160f gene from common wild rice and transferred into Arabidopsis thaliana to identify its function. The results showed that over-expression of miR160f decreased the number of rosette leaves, shortened bloting time, leading to early flowering. RT-PCR showed the expressions of gene ARF10,ARF16 and ARF17 are down-regulation caused by miR160f in transgenic Arabidopsis thaliana, while the deficiency of ARF10 and ARF16 protein can inhibit root cap cell differentiation, lose control of cell division and lead to ectopic expansion of apical stem cell populations Therefore, the result demonstrates that miR160 not only influence on root development, but also may influence flowering time of common wild rice.

Key words: MiRNA , Flowering time, Common wild rice, Arabidopsis thaliana , Expression vector

Yang Songnan,Wang Jiao,Chen Zongxiang,Tian Xinjie,Zhang Jingwen,Long Yan,Pei Xinwu,Yuan Qianhua. Clone and Function Analysis MiR160f in Common Wild Rice(Oryza rufipogon Griff.)[J]. Biotechnology Bulletin, 2014, 0(11): 114-118.

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