The Expression of Cathepsin B in the Different Ovary Development Stages of Marsupenaeus japonicus

Abstract

Abstract: The expression of Cathepsin B is tested in the different ovary development stages of Marsupenaeus japonicus by Real-time PCR. The results show Cathepsin B is in the highest level in the previtellogenic stage（PR）, medium level in the endogenous vitellogenic stage（EN）and exogenous vitellogenic stage（EX）. However the expression suddenly declines and is in the lowest level in the maturing stage（MA）. Cathepsin B of PR has significant difference compared with that of EN, EX and MA respectively（P<0.05）. There is no significant difference between EN and EX stages（P>0.05）. The expression of Cathepsin B in MA is significantly different from that in other three stages（P<0.05）. The results of Real-time PCR indicate that Cathepsin B may play one important role in the ovary development of Marsupenaeus japonicus.

Key words: Marsupenaeus japonicas, Ovary development, Cathepsin B, Real-time PCR , Gene expression

Cheng Cheng ,Lin Peng. The Expression of Cathepsin B in the Different Ovary Development Stages of Marsupenaeus japonicus [J]. Biotechnology Bulletin, 2014, 0(11): 159-163.

References

[1] 尹向辉, 程宝平, 刘维宾.日本对虾养殖技术讲座（一）—日本对虾的生物学特征[J].农村养殖技术, 2002, 9：18.
[2] 邱高峰.虾蟹类遗传育种学研究[J].水产学报, 1998, 22（3）：265-274.
[3]Benzie JA. Penaeid genetics and biotechnology[J]. Aquaculture, 1998, 164（1）：23-47.
[4]Preechaphol R, Leelatanawit R, SittikankeawI K, et al. Expressed sequence tag analysis for identification and characterization of sex-related genes in the giant tiger shrimp Penaeus monodon[J]. Journal of Biochemistry and Molecular Biology, 2007, 40（4）：501-510.
[5]Klinbunga S, Preechaphol R, Thumrungtanakit S, et al. Genetic diversity of the giant tiger shrimp（Penaeus monodon）in Thailand revealed by PCR-SSCP of polymorphic EST-derived markers[J]. Biochemical Genetics, 2006, 44（5-6）：222-236.
[6] Zhao XF, An XM, Wang JX, et al. Expression of the Helicoverpa ca-thepsin b-like proteinase during embryonic development[J]. Arch-ives of Insect Biochemistry and Physiology, 2005, 58（1）：39-46.
[7]Gerhartz B, Kolb HJ, Wittmann J. Proteolytic activity in the yolk sac membrane of quail eggs[J]. Comparative Biochemistry and Physiology Part A：Molecular & Integrative Physiology, 1999, 123（1）：1-8.
[8] Cho WL, Tsao SM, Hays AR, et al. Mosquito cathepsin B-like protease involved in embryonic degradation of vitellin is produced as a latent extraovarian precursor[J]. Journal of Biological Chemistry, 1999, 274（19）：13311-13321.
[9] Carnevali O, Carletta R, Cambi A, et al. Yolk formation and degrada-tion during oocyte maturation in seabream Sparus aurata：involve-ment of two lysosomal proteinases[J]. Biology of Reproduction, 1999, 60（1）：140-146.
[10] Kestemont P, Cooremans J, Abi-Ayad A, et al. Cathepsin L in eggs and larvae of perch Perca fluviatilis：variations with developmental stage and spawning period[J]. Fish Physiology and Biochemistry, 1999, 21（1）：59-64.
[11]Kirkland PA, Busby J, Stevens JRS, et al. Trizol-based method for sample preparation and isoelectric focusing of halophilic proteins[J]. Analytical Biochemistry, 2006, 351（2）：254-259.
[12] Kawazoei C, Jasmani SA, Shih TUW, et al. Purification and characterization of vitellin from the ovary of kuruma prawn, Penaeus japonicus[J]. Fisheries Science, 2000, 66（2）：390-396.
[13] Okumura T, Sakiyama K. Hemolymph levels of vertebrate—type steroid hormones in female kuruma prawn Marsupenaeus japonicus（Crustacea：Decapoda：Penaeidae）during natural reproductive cycle and induced ovarian development by eyestalk ablation[J]. Fisheries Science, 2004, 70（3）：372-380.
[14]Okumura T, Yamano K, Sakiyama K. Vitellogenin gene expression and hemolymph vitellogenin during vitellogenesis, final maturation, and oviposition in female kuruma prawn, Marsupenaeus japonicus[J]. Comparative Biochemistry and Physiology-Part A：Molecular & Integrative Physiology, 2007, 147（4）：1028-1037.
[15]Sellars MJ, Vuocolo T, Leeton LA, et al. Real-time RT-PCR quantification of Kuruma shrimp transcripts：a comparison of relative and absolute quantification procedures[J]. Journal of Biotechnology, 2007, 129（3）：391-399.
[16]Kim YK, Tsutsui N, Kawazoe I, et al. Localization and developme-ntal expression of mRNA for cortical rod protein in kuruma prawn Marsupenaeus japonicus[J]. Zoolog Sci, 2005, 22（6）：675-680.
[17] Kim YK, Kawazoe I, Tsutsui N, et al. Isolation and cDNA cloning of ovarian cortical rod protein in Kuruma prawn Marsupenaeus japon-icus（Crustacea：Decapoda：Penaeidae）[J]. Zoological Science, 2004, 21（11）：1109-1119.
[18] Seydoux G. Mechanisms of translational control in early developm-ent[J]. Current Opinion in Genetics & Development, 1996, 6（5）：555-561.
[19]Picton H, Briggs D, Gosden R. The molecular basis of oocyte growth and development[J]. Molecular and Cellular Endocrinology, 1998, 145（1-2）：27-37.
[20]Donald K, Day A, Smerdon G, et al. Quantification of gene transc-ription and enzyme activity for functionally important proteolytic enzymes during early development in the Pacific oyster Crassostrea gigas[J]. Comparative Biochemistry and Physiology Part B：Biochemistry and Molecular Biology, 2003, 136（3）：383-392.
[21] 淳俊, 郑彦峰, 王胜华, 等.一种广泛适用的RNA提取方法[J].生物化学与生物物理进展, 2008, 35（5）：591-597.