Screening and Identification of Streptomyces fungicidicus Strain AL-04

Abstract

Abstract: 13 actinomycetes strains were screened from soil samples of Qinghai-Tibet Plateau. Strain AL-04 which has high and stable inhibition effects on soilborne pathogens was selected by methods of agar diffusion, relative growth rate, spore germination inhibition and vegetable leaves. Results of antagonistic activity showed that AL-04 had strong antagonistic activity against 4 species of soilborne pathogens including Phytophthora capsici, Fusarium oxysporum f. sp. cucumerinum, Fusarium oxysporum f. sp. niveum and Botrytis cinerea. The inhibition rates were beyond 70.0% for all pathogens, of which 93.0% was observed for Phytophthora capsici. AL-04 was identified as Streptomyces fungicidicus by the observation of the morphological features, cytochemical characters and 16S rDNA sequence.

Key words: Actinomycetes Biocontrol, Biocontrol efficacy, Identification 16S, RDNA

Li Zengbo, Sun Hongyan, Zhao Jingxian, Zhou Jianwu, Liang Dong, Gao Ye. Screening and Identification of Streptomyces fungicidicus Strain AL-04[J]. Biotechnology Bulletin, 2014, 0(5): 155-161.

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