Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (6): 67-74.

• Technology and methods • Previous Articles     Next Articles

The Application of Uncultured Methods in the Study of Ruminal Methanogen Population

Wang Xingwen1,2,Wang Jiaqi1,Zhao Shengguo1,Li Fadi2,Bu Dengpan1   

  1. (1. State Key Laboratory of Animal Science, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193; 2. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070)
  • Received:2013-09-03 Online:2014-06-25 Published:2014-06-25

Abstract: The ruminal methanogen are strictly anaerobic, which are hard to culture.The ruminal methanogen which have been cultured successfully are only 4 species.while hundreds of species of methanogen could not be cultured.The application and development of uncultured technology promotes the research of ruminal methanogen, which overcome the limitation of the tranditional technology of isolation and culturing. Several dominant culture-independent technologies used to study ruminal methanogens were introduced in this paper to provide references on methods for future ruminal methanogen study.

Key words: Rumen, Methanogen, Uncultured methods, Whole genome sequencing, High throughput, DNA sequencing