Genetic Analysis and Molecular Mapping of Stripe Rust Resistance Gene in Wheat Line CH5383

Abstract

Abstract: CH5383 is an introgression lines from Thinopyrum intermedium with high resistance to wheat stripe rust and powdery mildew. The evaluations of disease were tested using Pst races CYR31 and CYR32 in seedlings. The result showed that the stripe rust resistance came from Thinopyrum intermedium in CH5383. Genetic analysis revealed that the resistance was controlled by a single dominant gene inoculating Pst race CYR32 inadult stage. It was temporarily named YrCH5383. Two polymorphic SSR markers, Xgwm108, Xbarc206 and Xbarc77 were linked to the resistance gene with genetic distance 8.2 cM, 10.7 cM and 13.6 cM, respectively. Based on marker loci and the origination, YrCH5383 might be a new gene to wheat stripe rust on 3BL.

Key words: Wheat, Stripe rust, Thinopyrum intermedium, Genetic analysis, Molecular mapping

Zhan Haixian,Chang Zhijian,Li Guangrong,Jia Juqing,Guo Huijuan,Zhang Xiaojun,Li Xin,Qiao Linyi,Yang Zujun. Genetic Analysis and Molecular Mapping of Stripe Rust Resistance Gene in Wheat Line CH5383[J]. Biotechnology Bulletin, 2014, 0(6): 96-100.

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