Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (8): 132-137.

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Molecular Identification of 23 Marine Fungal Strains and Their Activities Against Plant Pathogenic Fungi and Cytotoxic Activities

Yang Xiaolan1,2,3, Chen Yuchan2, Li Haohua2, Zhang Weimin2   

  1. 1. South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301;
    2. State Key Laboratory of Applied Microbiology, South Chinathe Ministry-Province Joint Development, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou 510070;
    3. University of Chinese Academy of Sciences, Beijing 100049
  • Revised:2013-12-18 Online:2014-08-15 Published:2014-08-01
  • Contact: 通讯作者: 章卫民，男，博士，研究员，研究方向：药用微生物资源及其活性物质；E-mail：wmzhang58@qq.com
  • Supported by:

    国家自然科学基金项目(31272087);国家“863”计划资助项目(2012AA092104);广东省自然科学基金项目(S2013010014557);广州市科技计划项目(2013J4100067)

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Abstract:

Twenty three marine fungal strains were isolated from the sediments of the South China Sea and identified by intergenic transcribed spacer（ITS）sequencing. The fermentation broth extracts of these marine fungal strains were tested for their inhibitory activities against growth of 4 plant pathogenic fungi, namely Colletotrichum gloeosporioides, Alternaria alternate, Curvularia lunata, Cylindrocladium scoparium, and investigated for their cytotoxic activities against SF-268, MCF-7, NCI-H460 and HepG-2 tumor cell lines by the SRB method. The results showed the extracts of 11 strains presented higher than 50% inhibitory rates against at least one of those plant pathogenic fungi at a concentration of 50 mg/mL and the extracts of 9 strains presented higher than 80% inhibitory rates against at least one of those tumor cell lines at a concentration of 100 μg/mL. Among all strains studied, Eupenicillium sp. FS100, Penicillium sp. FS105, Dichotomomyces cejpii FS110 and Acaromyces ingoldii FS121 exhibited obvious inhibitory activities against plant pathogenic fungi and/or tumor cell lines.

Key words: Marine fungi, Molecular identification, Extract, Activity against plant pathogenic fungi, Cytotoxicity