Screen High-producing Laccase Strain of Bjerkandera fumosa Through UV and 60Co Composite Mutagenesis

Abstract

Abstract: Mutagenic strains ZW-7 was obtained from the spore suspension of Bjerkandera fumosa（5.0172）by UV mutagenesis and screening. The laccase activity was 1.33 times than the original strain. After subcultured five generations, laccase activity was stable. ZW-7 was mutagenized to a new mutagenic strain Co-11 by treating with 60Co-γ ray radiation and its activity was 380.5 U/L, which was 1.18 times than ZW-7 and 1.58 times than the original strain（250.6 U/L）. After subcultured five generations, enzyme activities of Co-11 remains stable.

Key words: Bjerkandera fumosa , UV, 60Co-γ rays, Mutagenic Laccase

Peng Yanchao, Cao Fuxiang,Dong Xujie, Peng Jiqing, Hu Shuangxi. Screen High-producing Laccase Strain of Bjerkandera fumosa Through UV and 60Co Composite Mutagenesis[J]. Biotechnology Bulletin, 2014, 0(9): 120-124.

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